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| 供应商 | 上海联迈生物工程有限公司 |
| 数量 | 大量 |
| 目录编号 | LM-0812R |
| 克隆性 | 多克隆 |
| 抗原来源 | Rabbit |
| 保质期 | 1年 |
| 抗体英文名 | IL-1 Beta |
| 抗体名 | 白介素1β/IL-1β抗体 |
| 宿主 | Rabbit |
| 适应物种 | Human, Mouse, Rat, Dog, Pig, Rabbit, Guinea Pig, |
| 免疫原 | KLH conjugated synthetic peptide derived from human IL-1beta:101-200/269 |
| 亚型 | IgG |
| 形态 | Lyophilized or Liquid |
| 应用范围 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） |
| 浓度 | 1mg/ml |
| 保存条件 | Store at -20 °C |
| 规格 | 50ul 100ul 200ul |
| 英文名称 | IL-1 Beta |
| 中文名称 | 白介素1β/IL-1β抗体 |
| 别 名 | Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta. IL-1B |
 | Specific References (12) | bs-0812R has been referenced in 12 publications. [IF=2.68] Wang, Hui, et al. "Anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in a rabbit ear model." European Journal of Pharmacology (2015). IHC-P ; Rabbit. PubMed:25661977 [IF=1.48] Li, Kang, et al. "CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts." Molecular Medicine Reports. other ; PubMed:26081375 [IF=6.03] Johann, Sonja, et al. "NLRP3 inflammasome is expressed by astrocytes in the SOD1 mouse model of ALS and in human sporadic ALS patients." Glia (2015). WB ; Human. PubMed:26200799 [IF=2.46] de Jesus, Julio Fernandes, et al. "Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon." Lasers in Medical Science 30.1 (2015): 153-158. IHC-P ; Rat. PubMed:25070591 [IF=1.35] Hu, Ruiqi, et al. "Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period."Acta histochemica 118.2 (2016): 164-169. Other Species. PubMed:26746863 [IF=1.35] Hu, Ruiqi, et al. "Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period."Acta histochemica 118.2 (2016): 164-169. IHC-P ; Other Species. PubMed:26746863 [IF=1.35] Xi, Liqin, et al. "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." Acta Histochemica (2016). IHC-P ; Other Species. PubMed:27919431 [IF=5.40] Heitzer, Marius, et al. "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1 (G93A) ALS Mice." Molecular Neurobiology: 1-15. WB ; Mouse. PubMed:27957680 [IF=1.06] Liu, Dong-qing, Xiao-jing Li, and Xiao-juan Weng. "Effect of BTXA on Inhibiting Hypertrophic Scar Formation in a Rabbit Ear Model." Aesthetic Plastic Surgery (2017): 1-8. IHC-P ; Rabbit. PubMed:28180922 [IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166. WB ; Mouse. PubMed:26781175 [IF=2.03] Zhong, Shigen, et al. "Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction." Ultrasonics 52.2 (2012): 281-286. WB ; PubMed:21937069 [IF=2.15] Bagriyanik, H. A., et al. "The effects of resveratrol on chronic constriction injury of sciatic nerve in rats." Neuroscience Letters (2014). IHC-P ; Rat. PubMed:24394911 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 糖蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Rabbit, Guinea Pig, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-1beta:101-200/269 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Subunit: Monomer. Subcellular Location: Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Similarity: Belongs to the IL-1 family. SWISS: P01584 Gene ID: 3553 Database links: Entrez Gene: 3553 Human Entrez Gene: 16176 Mouse Entrez Gene: 24494 Rat Omim: 147720 Human SwissProt: P01584 Human SwissProt: P10749 Mouse SwissProt: Q63264 Rat Unigene: 126256 Human Unigene: 222830 Mouse Unigene: 9869 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 生长因子和激素（ Growth Factor and Hormones） IL-1β是一种糖蛋白，它既可诱导急性期时相关蛋白的产生，也是β细胞终末分化并分泌抗体的必需因子，在免疫调节中发挥重要做 用。 IL-1β前体在细胞内合成后被酶剪切为成熟形态后排出到细胞外,IL-1β的前体有153个氨基酸。成熟形态有116个氨基酸。 |
| 产品图片 |  Sample: Raw246.7 (Mouse)Cell Lysate at 40 ug Primary: Anti- IL-1 beta (bs-0812R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 30 kD  Tissue/cell: Human cervical carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated(bs-0812R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated(bs-0812R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated(bs-0812R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control (black line): A431(black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (green line): Rabbit Anti-PTP1B antibody (bs-3152R) ; Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test. |
