重组人神经源性丝氨酸蛋白酶抑制剂（rHuNeuroserpin）Recombinant
Human Neuroserpin
(rHuNeuroserpin)
PrimeGene Technical Data Sheet
Catalog Number:
402-02
Source:
Escherichia coli.
Molecular Weight:
Approximately 44.8kDa, a single non-glycosylated polypeptide chain containing 395 amino acid
residues.
Quantity:
5μg/25μg/1000μg
AA Sequence:
MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ
GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ
NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD
LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP
MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA
TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI
FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV
LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL
Purity:
>95% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The ED
50
determined by a cell proliferation
assay using rat C6 cells is less than 600 ng/ml, corresponding to a specific activity of >1.67×10
3
IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2
μ
敲敤湣敮潬楯偂匬⁰‵‵‵⸴
Endotoxin:
Less than 1EU/
μ
g of rHuNeuroserpin as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20
°
C. Further dilutions should be made in appropriate buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8
°
C, but should be kept at -20
°
C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8
°
C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20
°
C to
-70
°
C.
Avoid repeated freeze/thaw cycles
.
Usage:
This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further
evaluation purposes.
NOT FOR HUMAN USE
.
Human Neuroserpin
Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological targe
t
of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degrada
tion
of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. The neuroprotective
role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in
these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin,
caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with
neuroserpin inclusion bodies (FENIB).
