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| Identity (IR-spectrum) | conforms |
| In water insoluble matter | ≤ 0.005 % |
| Absorbance (290 nm; 40 %; 1 cm; water) | ≤ 0.2 |
| Chloride (Cl) | ≤ 0.001 % |
| Sulfate (SO₄) | ≤ 0.005 % |
| Heavy metals (as Pb) | ≤ 0.0005 % |
| Fe (Iron) | ≤ 0.0005 % |
| Sulfated ash | ≤ 0.05 % |
| Water (according to Karl Fischer) | ≤ 0.5 % |
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- as a buffer component in decellularization of tissues
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Specifications
DNAse: none detected
RNAse: none detected
Protease: none detected
Arsenic (As): ≤0.0005%
Copper (Cu): ≤0.0001%
Iron (Fe): ≤0.0001%
Lead (Pb): ≤0.0001%
Magnesium (Mg): ≤0.0005%
Loss on drying: ≤1%
A280 (1 M, water): ≤0.05 AU
Purity: ≥99.8% by assay
Solubility: 10% in water
Insoluble matter: ≤0.005%
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- Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
- Investigating the influence of solvent type and pH on protein adsorption onto silica surface by evanescent-wave cavity ring-down spectroscopy.: This study uses TRIS Hydrochloride to explore protein-silica interactions, providing insights into surface chemistry and biomolecular adsorption processes (Alnaanah & Mendes, 2024).
',description_type_id:3},{label:"Disclaimer",value:"Toxicity: Irritant (B)",description_type_id:11},{label:"General description",value:"Molecular Biology Grade.
260 (10%, water): ≤0.05 AU
A280 (10%, water): ≤0.05 AU
Assay: ≥99%
DNAse: none detected
Identity IR spectrum: conforms
Lead (Pb): ≤.0002%
Phosphatase: none detected
Protease: none detected
RNAse: none detected
Water (H2O): ≤0.5%
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.",description_type_id:16},{label:"Other Notes",value:"The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.",description_type_id:20},{label:"Legal Information",value:"CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany",description_type_id:22},{label:"Legal Information",value:"OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany",description_type_id:22},{label:"Legal Information",value:"Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany",description_type_id:22}],related_products:[],product_categories:[{category:"Biochemicals",url:"/products/chemistry-and-biochemicals/biochemicals"},{category:"Biological Buffers",url:"/products/chemistry-and-biochemicals/biochemicals/biological-buffers"}],links:[{image:"/content/dam/sigma-aldrich/head/search/structure-icon.gif",label:"Structure Search",url:'Structure Search',key:"structure_search",seq:25}],forms:[{type:"bulk order",label:"Request a Bulk 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- as an assay buffer for reconstitution of extracted and dried protein samples
- to prepare Tris-HCl buffer that is used to stabilize proteins
- as a buffer to extract carotenoid from tubers
- as a component of sample buffer during protein extraction prior to western blotting
- as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
- as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
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- 25KG-DP are the standard prepack offering
- Smaller volumes can be put in the same drum for variable configurations
The benefits of DRYPOUR®: - Improves operational efficiency by reducing material preparation time and increasing operator safety
- Minimizes caking of hygroscopic salts
- Offers triple protection: against moisture inside and outside, and against contamination
How does the DRYPOUR® packaging system Improve your operational efficiency? - This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
- PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek® liner
- Desiccant bags are firmly integrated into the polyethylene liner
- Triple protection delivers two invaluable results:
- Dramatically reduced caking
- No contamination risk from the desiccant
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clinical testing
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- As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
- As a component of pre-hybridization buffer in in situ hybridization
- To suspend dried protein before SDS-PAGE protein characterization
- As a component of lysis buffer to wash and lyse cells before immunoprecipitation
- In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
- as a component of Tris/base solution in a sulforhodamine B (SRB) assay
',description_type_id:3},{label:"Features and Benefits",value:"- Suitable for Cell culture, Biochemical and Molecular Biology Research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 7-9 (25 \xb0C) with a pKa of 8.1 (25 \xb0C)
- Tested for the presence of Anionic and Cationic traces
- Tested for DNase, RNase, and protease
",description_type_id:14},{label:"General description",value:"Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes.
This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic.
In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation.",description_type_id:16},{label:"Other Notes",value:'For additional information on our range of Biochemicals, please complete this form.',description_type_id:20},{label:"Other Notes",value:"The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per \xb0C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.",description_type_id:20},{label:"Packaging",value:"T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.",description_type_id:21},{label:"Legal Information",value:"Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany",description_type_id:22}],related_products:[{pid:"T5941SIGMA",rs_type:"related product"},{pid:"A9099SIGMA",rs_type:"related product"},{pid:"E5134SIGMA",rs_type:"related product"},{pid:"G8898SIGMA",rs_type:"related product"},{pid:"T6687SIGMA",rs_type:"comparable product"},{pid:"T6791SIGMA",rs_type:"comparable product"},{pid:"T1378SIGMA",rs_type:"comparable product"},{pid:"T1503SIGMA",rs_type:"comparable product"},{pid:"T4661SIGMA",rs_type:"comparable product"},{pid:"93350SIGMA",rs_type:"comparable product"},{pid:"93352SIGMA",rs_type:"comparable product"},{pid:"93362SIGMA",rs_type:"comparable product"},{pid:"33742SIAL",rs_type:"comparable product"},{pid:"154563ALDRICH",rs_type:"comparable product"},{pid:"252859SIAL",rs_type:"comparable product"},{pid:"T1699SIGMA",rs_type:"comparable 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