LOCUS p04 8348 bp DNA circular 22-AUG-2017 DEFINITION . ACCESSION urn.local...2e-7x36jdy KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 35..430 /label="U6gRNA" misc_feature 82..101 /label="gRNA SeqPrimer" misc_feature 432..459 /label="Vector Tag" misc_feature 463..1042 /label="CMV" misc_feature 1093..1112 /label="T7" misc_feature 1141..1206 /label="3xFlag" misc_feature 1207..5307 /label="eCas91.1" misc_feature 5308..5328 /label="NLS" misc_feature 5335..5403 /label="2A" misc_feature 5410..6123 /label="GFP" polyA_signal 6179..6403 /label="BGH pA" misc_feature 6361..6366 /Recognition_pattern="GAAGAC(2/6)" /label="BbsI" /note="Geneious type: restriction site" misc_feature 6576..7370 /label="KanR" rep_origin 7669..8342 /label="pUC ori" ORIGIN 1 gctgcttcgc gatgtacggg ccagatatac gcgtaaggtc gggcaggaag agggcctatt 61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat 121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt 181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt 241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc 301 aggtggacac ctgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 361 caacttgaaa aagtggcacc gagtcggtgc ttttttctag gcccagcttt cttgtacaaa 421 gttggcatta gtcgacaagc acagctgagc aaccttaatt aatgacattg attattgact 481 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 601 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 661 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 781 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 841 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 901 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 961 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1021 cggtgggagg tctatataag caaccaggtt ctggctaact agagaaccca ctgcttactg 1081 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc cgccaccatg 1141 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac 1201 gataaggaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc 1261 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac 1321 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc 1381 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc 1441 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac 1501 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc 1561 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga 1621 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc 1681 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc 1741 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac 1801 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc 1861 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga 1921 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc 1981 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg 2041 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc 2101 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc 2161 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg 2221 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac 2281 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc 2341 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg 2401 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg 2461 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag 2521 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac 2581 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag 2641 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat 2701 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc 2761 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc 2821 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg 2881 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa 2941 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa 3001 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc 3061 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat 3121 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg 3181 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc 3241 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac 3301 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc 3361 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag 3421 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc 3481 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag 3541 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac 3601 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg 3661 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac 3721 catatcgtgc ctcagagctt tctggcggac gactccatcg acaacaaggt gctgaccaga 3781 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg 3841 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat 3901 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga 3961 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg 4021 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag 4081 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac 4141 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa 4201 aagtaccctg cgctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg 4261 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac 4321 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag 4381 gcgcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat 4441 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag 4501 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg 4561 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg 4621 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt 4681 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc 4741 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct 4801 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa 4861 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc 4921 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg 4981 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga 5041 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat 5101 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga 5161 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc 5221 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg 5281 atcgacctgt ctcagctggg aggcgacccc aagaaaaagc gcaaagtggt taacggcagt 5341 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccacga 5401 tcgatgcata gcgggggcga ggagctgttc gccggcatcg tgcccgtgct gatcgagctg 5461 gacggcgacg tgcacggcca caagttcagc gtgcgcggcg agggcgaggg cgacgccgac 5521 tacggcaagc tggagatcaa gttcatctgc accaccggca agctgcccgt gccctggccc 5581 accctggtga ccaccctctg ctacggcatc cagtgcttcg cccgctaccc cgagcacatg 5641 aagatgaacg acttcttcaa gagcgccatg cccgagggct acatccagga gcgcaccatc 5701 cagttccagg acgacggcaa gtacaagacc cgcggcgagg tgaagttcga gggcgacacc 5761 ctggtgaacc gcatcgagct gaagggcaag gacttcaagg aggacggcaa catcctgggc 5821 cacaagctgg agtacagctt caacagccac aacgtgtaca tccgccccga caaggccaac 5881 aacggcctgg aggctaactt caagacccgc cacaacatcg agggcggcgg cgtgcagctg 5941 gccgaccact accagaccaa cgtgcccctg ggcgacggcc ccgtgctgat ccccatcaac 6001 cactacctga gcactcagac caagatcagc aaggaccgca acgaggcccg cgaccacatg 6061 gtgctcctgg agtccttcag cgcctgctgc cacacccacg gcatggacga gctgtacagg 6121 gctgttaact gatgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact 6181 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 6241 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 6301 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 6361 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctac tgggcggttt 6421 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc 6481 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa 6541 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 6601 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 6661 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 6721 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 6781 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 6841 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 6901 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 6961 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 7021 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 7081 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 7141 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 7201 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 7261 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 7321 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg 7381 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 7441 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 7501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac 7561 gtgctaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 7621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 7681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 7741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 7801 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 7861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 7921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 7981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 8041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 8101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 8161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 8221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 8281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 8341 atgttctt //