Proteline
链霉蛋白酶(Pronase E)
CAS: 9036-06-0
化学式:
| 中文名 | 链霉蛋白酶 |
| 英文名 | Pronase E |
| 别名 | 链酶蛋白酶 链霉蛋白酶 蛋白质水解酶 链霉蛋白酶E 蛋白酶 XIV 链丝菌蛋白酶E 蛋白酶 来源于灰色链霉菌 链丝菌蛋白酶E(灰色链霉菌) |
| 英文别名 | Proteline Pronase E EC 3.4.24.4 s.Griseusproteinase Streptomycesgriseusprotease pronasef.streptomycesgriseus Proteinase,streptomycesgriseus ACTINASE E, STREPTOMYCES GRISEUS Protease from Streptomyces griseus,Actinase E, Pronase E |
| CAS | 9036-06-0 |
| EINECS | 232-909-5 |
| 溶解度 | 溶于水等。 |
| 存储条件 | 2-8°C |
| 外观 | 粉末 |
| 颜色 | slightly brown |
| 物化性质 | 理化性质 白色或微褐色粉末。链霉蛋白酶是从灰色链霉菌(Streptomyces griseus)中分离出的一种非特异蛋白水解酶(Protease)的专有名称。分子量一般为20000。易溶于盐水和稀盐溶液，最适pH值7.8～8.0 |
| MDL号 | MFCD00132092 |
| 危险品标志 | Xn - 有害物品 |
| 风险术语 | R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 R42 - 吸入可能致敏。 |
| 安全术语 | S22 - 切勿吸入粉尘。 S24 - 避免皮肤接触。 S26 - 不慎与眼睛接触后，请立即用大量清水冲洗并征求医生意见。 S36/37 - 穿戴适当的防护服和手套。 S24/25 - 避免与皮肤和眼睛接触。 |
| WGK Germany | 1 |
| RTECS | UK9540000 |
| FLUKA BRAND F CODES | 10 |
| 参考资料 展开查看 | 1. 胡文彬, 贾彦博, 魏琴芳,等. 应用液相色谱-原子荧光联用仪测定富硒大米中的5种硒形态[J]. 分析仪器, 2019, 222(01):126-130. 2. 魏琴芳, 贾彦博, 胡文彬,等. 液相色谱-电感耦合等离子质谱联用法测定大豆中的5种硒形态[J]. 食品安全质量检测学报, 2020, 011(008):2456-2461. 3. 王铁良, 张会芳, 魏亮亮,等. 高效液相色谱-氢化物发生-原子荧光光谱联用技术测定富硒大米中的5种硒形态[J]. 食品安全质量检测学报, 2017(06):249-254. 4. 刘涵 尹子楚 蔡玉荣 等. 丝素蛋白/多巴胺医用粘合剂的制备及其性能[J]. 浙江理工大学学报 2020 043(003):308-314. 5. 黄瑾,吴丽莉,徐仲玉.基于药物亲和反应靶标稳定性技术的综合型实验探索[J].实验室研究与探索,2020,39(11):220-223+261. 6. Lin, Dongju, et al. "Lysine-and cysteine-based protein adductions derived from toxic metabolites of 8-epidiosbulbin E acetate." Toxicology letters 264 (2016): 20-28.http://doi.org/10.1016/j.toxlet.2016.10.007 7. Li, Weiwei, et al. "Lysine adduction by reactive metabolite (s) of monocrotaline." Chemical research in toxicology 29.3 (2016): 333-341.http://doi.org/10.1021/acs.chemrestox.5b00488 8. Li, Qingmei, et al. "Glutathione conjugation and protein adduction by environmental pollutant 2, 4-dichlorophenol in vitro and in vivo." Chemical Research in Toxicology 33.9 (2020): 2351-2360.http://doi.org/10.1021/acs.chemrestox.0c00118 9. Wang, Kai, et al. "Detection of cysteine-and lysine-based protein adductions by reactive metabolites of 2, 5-dimethylfuran." Analytica chimica acta 896 (2015): 93-101.http://doi.org/10.1016/j.aca.2015.09.017 10. Wang, Hui, et al. "Cysteine-based protein adduction by epoxide-derived metabolite (s) of benzbromarone." Chemical research in toxicology 29.12 (2016): 2145-2152.10.1021/acs.chemrestox.6b00275 11. Wang, Kai, et al. "Chemical identity of interaction of protein with reactive metabolite of Diosbulbin B in vitro and in vivo." Toxins 9.8 (2017): 249.http://doi.org/10.3390/toxins9080249 12. Chen, Yi, et al. "Anti-thrombotic and pro-angiogenic effects of Rubia cordifolia extract in zebrafish." Journal of ethnopharmacology 219 (2018): 152-160.http://doi.org/10.1016/j.jep.2017.11.005 13. [IF=6.558] Kai Wang et al."Detection of cysteine- and lysine-based protein adductions by reactive metabolites of 2,5-dimethylfuran."Anal Chim Acta. 2015 Oct;896:93 14. [IF=4.658] Baoyin Zhao et al."Intramyocardial transplantation of cardiac telocytes decreases myocardial infarction and improves post‐infarcted cardiac function in rats."J Cell Mol Med. 2014 May;18(5):780-789 15. [IF=4.546] Kai Wang et al."Chemical Identity of Interaction of Protein with Reactive Metabolite of Diosbulbin B In Vitro and In Vivo."Toxins. 2017 Aug;9(8):249 16. [IF=4.372] Dongju Lin et al."Lysine- and cysteine-based protein adductions derived from toxic metabolites of 8-epidiosbulbin E acetate."Toxicol Lett. 2016 Dec;264:20 17. [IF=4.36] Yi Chen et al."Anti-thrombotic and pro-angiogenic effects of Rubia cordifolia extract in zebrafish."J Ethnopharmacol. 2018 Jun;219:152 18. [IF=4.192] Mengmeng Zhang et al."Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide from Lepidium meyenii."J Agr Food Chem. 2016;64(9):1921–1931 19. [IF=3.739] Weiwei Li et al."Lysine Adduction by Reactive Metabolite(s) of Monocrotaline."Chem Res Toxicol. 2016;29(3):333–341 20. [IF=3.739] Xiucai Guo et al."Electrophilicities and Protein Covalent Binding of Demethylation Metabolites of Colchicine."Chem Res Toxicol. 2016;29(3):296–302 21. [IF=3.739] Hui Wang et al."Cysteine-Based Protein Adduction by Epoxide-Derived Metabolite(s) of Benzbromarone."Chem Res Toxicol. 2016;29(12):2145–2152 22. [IF=3.031] Zhang Wen-Juan et al."NLRP3 inflammasome activation from Kupffer cells is involved in liver fibrosis of Schistosoma japonicum- infected mice via NF-κB."Parasite Vector. 2019 Dec;12(1):1-18 23. [IF=4.36] Mingliang Gao et al."Evaluation of VEGF mediated pro-angiogenic and hemostatic effects and chemical marker investigation for Typhae Pollen and its processed product."J Ethnopharmacol. 2021 Mar;268:113591 24. [IF=4.152] Wei Zhang et al."Cell-Free Biomimetic Scaffold with Cartilage Extracellular Matrix-Like Architectures for In Situ Inductive Regeneration of Osteochondral Defects."Acs Biomater Sci Eng. 2020;6(12):6917–6925 25. [IF=3.922] Hui Wang et al."Glutathione Conjugation and Protein Adduction Derived from Oxidative Debromination of Benzbromarone in Mice."Drug Metab Dispos. 2019 Nov;47(11):1281-1290 26. [IF=3.739] Qingmei Li et al."Glutathione Conjugation and Protein Adduction by Environmental Pollutant 2,4-Dichlorophenol In Vitro and In Vivo."Chem Res Toxicol. 2020;33(9):2351–2360 27. [IF=11.236] Meiling Liu et al."Cercosporin-bioinspired photoinactivation of harmful cyanobacteria under natural sunlight via bifunctional mechanisms."Water Res. 2022 May;215:118242 |
Proteline - 介绍
&&最适PH：pH7.5。 &&最适温度40℃。 &&分子量：20000～60000。 &&性状：白色或微褐色粉末。 &&来源：灰色链霉菌。 Unite definition: The unit of non-specific protease activity is in general the enzyme activity that increases the rate of release of folin-positive amino acids and peptides from casein at a temperature of 40°C and pH 7.5 by a quantity equivalent to a given amount of tyrosine per minute. For the unit designated ‘U’, that quantity of tyrosine is 1 µmol/min
最后更新：2022-10-16 17:41:00
Proteline - 用途与合成方法
链霉蛋白酶（也称为酶链霉素酶）是一种重要的酶，用于分解和降解链霉素。链霉素是一种广谱抗生素，常用于治疗多种细菌感染。细菌的抗性发展，链霉素的有效性逐渐降低。
链霉蛋白酶的合成方法主要通过基因工程技术来实现。从链霉菌中提取链霉素酶的基因序列。然后，将该基因序列插入适当的表达载体中，该载体可以在大肠杆菌等细菌中进行高效表达。接下来，将带有链霉蛋白酶基因的载体转化到宿主细胞中，并进行培养。通过适当的诱导条件（如添加适量的诱导剂），细菌开始表达链霉蛋白酶。使用蛋白质纯化技术来纯化链霉蛋白酶，以得到高纯度的酶产物。
链霉蛋白酶的合成方法主要通过基因工程技术来实现。从链霉菌中提取链霉素酶的基因序列。然后，将该基因序列插入适当的表达载体中，该载体可以在大肠杆菌等细菌中进行高效表达。接下来，将带有链霉蛋白酶基因的载体转化到宿主细胞中，并进行培养。通过适当的诱导条件（如添加适量的诱导剂），细菌开始表达链霉蛋白酶。使用蛋白质纯化技术来纯化链霉蛋白酶，以得到高纯度的酶产物。
最后更新：2024-04-10 22:29:15
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