1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体 流式抗体
  4. Annexin-2/ANXA2 抗体 (YA619)

Annexin-2/ANXA2 抗体 (YA619)

目录号: HY-P80015
COA 抗体使用指南 技术支持

Annexin-2/ANXA2 Antibody (YA619) 是一个兔来源、无偶联标记、抗 Annexin-2/ANXA2 的 IgG 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μL ¥564 In-stock
50 μL ¥1464 In-stock
100 μL ¥2400 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Annexin-2/ANXA2 Antibody (YA619) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin-2/ANXA2.

宿主

Rabbit

克隆性

Recombinant,Monoclonal

分子量
Predicted band size: 39 kDa;
Observed band size: 34 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human Annexin A2.AA range:26-75.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:2000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-1:200
FC
FC: 流式细胞术
1:50-1:200
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB ICC
  • Western blot analysis of extracts from C6(lane 2(20ug) , NIH/3T3(lane 3(20ug) and RAW264.7(lane 4(20ug) using Annexin-2/ANXA2 Antibody (HY-P80015) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling Annexin-2/ANXA2 with Annexin-2/ANXA2 Antibody (HY-P80015) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Annexin-2/ANXA2 Antibody (HY-P80015) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of NIH/3T3 cells labeling Annexin-2/ANXA2 with Annexin-2/ANXA2 Antibody (HY-P80015) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Annexin-2/ANXA2 Antibody (HY-P80015) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:钙调节膜结合蛋白,其对钙的亲和力可被阴离子磷脂显著增强。它能以高亲和力结合两个钙离子。可能参与热应激反应。抑制 PCSK9 增强的 LDLR 降解,可能通过翻译机制降低 PCSK9 蛋白水平,但也会与 LDLR 竞争结合 PCSK9 (PubMed:18799458, PubMed:22848640, PubMed:24808179)。结合被颗粒磨损碎片吞噬作用损伤的内体,参与内体膜稳定,从而限制 NLRP3 炎症小体的激活 (基于相似性)。内皮细胞表面纤溶酶生成所必需,可能支持纤溶监视和新生血管生成 (基于相似性);(微生物感染) 结合肺炎支原体 CARDS 毒素,可能作为该病原体的受体之一。当 ANXA2 被 siRNA 下调时,毒素与人体细胞的结合减少,空泡化 (肺炎支原体感染的症状之一) 也随之减少。
亚细胞定位:分泌物、细胞外空间、细胞外基质、基底膜;黑素体
亚基:异源四聚体,包含 2 条 S100A10/p11 轻链和 2 条 ANXA2/p36 重链 (通过相似性)。
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
Annexin-2/ANXA2 抗体 (YA619)
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Annexin-2/ANXA2 Antibody (YA619)
目录号:
HY-P80015
需求量: