Bak 抗体 (YA592)

Bak Antibody (YA592) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bak.

Bak 是一种在线粒体凋亡过程中起关键作用的蛋白质。当细胞接收到死亡信号时，Bak 通过寡聚化在线粒体外膜 (MOM) 上形成孔道，促进 MOM 通透性改变。这一过程导致凋亡相关因子 (如细胞色素 c) 释放到细胞质中，进而激活 caspase 9，并最终促使效应 caspases 的激活。

Free

Q16611

Predicted band size: 23 kDa; Observed band size: 23 kDa

Protein A affinity purified.

Mitochondrion membrane.

Non-conjugated

Unmodified

AB_3102267

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:500; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg) , HEK293 (lane 4(20μg),using Bak Antibody (HY-P80026). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Bak with Bak Antibody (HY-P80026) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bak Antibody (HY-P80026) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Bak with Bak Antibody (HY-P80026) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bak Antibody (HY-P80026)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Bak.AA range:1-50.