BMAL1 抗体 (YA581)

BMAL1 Antibody (YA581) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BMAL1.

BMAL1 是一种转录激活因子，构成生物钟的核心组件。生物钟作为内部计时系统，通过调控基因表达产生约 24 小时的昼夜节律（circadian rhythm），进而影响代谢和行为。它由拉丁词根“circa”（大约）和“diem”（天）衍生而来，广泛调节生理功能，包括代谢、睡眠、体温、血压及内分泌、免疫、心血管和肾脏功能。生物钟分为位于大脑视交叉上核（SCN）的中枢时钟和存在于各组织器官的外周时钟，两者均可被环境 Zeitgeber（时间线索，如光信号）重置。中枢时钟通过神经/激素信号、体温和进食线索同步外周时钟，使其与外界光/暗周期对齐。BMAL1 与 CLOCK 或 NPAS2 形成异源二聚体，作为转录/翻译反馈环（TTFL）的正向调控元件，激活含 E-box（5'-CACGTG-3'）的时钟基因（如 PER1/2/3、CRY1/2）和代谢相关基因的转录。这些基因产物又反馈抑制 BMAL1 活性，形成负向调控。此外，BMAL1 通过调控 Wnt 通路基因抑制脂肪生成（adipogenesis）、促进肌生成（myogenesis）；影响胰腺 β 细胞功能及胰岛素分泌；负调 mTORC1 信号通路；调控单核细胞节律性募集和卵巢类固醇合成基因（如 CYP19A1）。在神经发生中，它调控神经干/祖细胞（NSPCs）的细胞周期进程。BMAL1 还通过染色质开放节律性调控其他转录因子的 DNA 可及性。其与 CLOCK 的异源二聚体偏好结合 5'-CACGTGA-3' 序列（含 E-box 侧翼腺嘌呤），并识别非经典 E-box 变体（如 5'-AACGTGA-3'）。在感染中，BMAL1 可能通过调控 ACE2 和干扰素刺激基因影响 SARS-CoV-2 的细胞入侵。相似功能（By similarity）包括：通过 PKM2 依赖性机制限制巨噬细胞中 CD274 表达以抵御败血症；通过激活 DGAT2 和 COQ10B 基因调控骨骼肌代谢节律。

Free

O00327

Predicted band size: 69 kDa; Observed band size: 70 kDa

Protein A affinity purified.

Nucleus, PML body, Cytoplasm.

Non-conjugated

Unmodified

AB_3102270

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2000; ICC/IF: 1:50-1:200

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) , Hela(lane 3(40μg) and NIH3T3(lane 4(20ug) , NIH3T3(lane 5(40ug) using BMAL1(HY-P80033 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling BMAL1 with BMAL1 Antibody (HY-P80033) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with BMAL1 Antibody (HY-P80033) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of SHSY5Y cells labeling BMAL1 with BMAL1 Antibody (HY-P80033) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with BMAL1 Antibody (HY-P80033)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human BMAL1.AA range:550-599.