E-Cadherin 抗体 (YA470)

E-Cadherin Antibody (YA470) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E-Cadherin.

Cadherins 是一类钙依赖性细胞粘附蛋白，优先以同源方式在连接细胞中相互作用，从而促进异质细胞类型的分类。CDH1 参与调控上皮细胞的细胞间粘附、迁移和增殖机制。通过与 AMOTL2 的相互作用（by similar），CDH1 促进径向肌动蛋白纤维结构的组织化，并介导细胞对收缩力的响应，协助将径向肌动蛋白纤维锚定到细胞膜上的 CDH1 连接复合物。在桥粒细胞间连接形成的早期阶段，CDH1 通过促进 DSG2 和 DSP 向桥粒斑块的募集发挥作用。它具有强效的侵袭抑制功能，是整合素 α-E/β-7 的配体；E-Cad/CTF2 促进 Aβ 前体的非淀粉样蛋白降解途径，并对 APP C99 和 C83 的产生具有显著抑制作用。此外（微生物感染），CDH1 可作为单核细胞增生李斯特菌的受体，其表面蛋白 internalin A（InlA）与 CDH1 结合并促进细菌的内化。

Free

P12830

Predicted band size: 97 kDa； Observed band size: 80~120 kDa

Protein A affinity purified.

Cell junction, Cell membrane, Endosome, Golgi apparatus.

Non-conjugated

Unmodified

AB_3102080

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from HT-29(lane 2(20μg), A431(lane 3(20μg) and HEK293(lane 4(20μg) using E-Cadherin (HY-P80113) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling E-Cadherin with E-Cadherin Antibody (HY-P80113)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with E-Cadherin Antibody (HY-P80113) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling E-Cadherin with E-Cadherin Antibody (HY-P80113) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with E-Cadherin Antibody (HY-P80113) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Flow cytometric analysis of 1X10^6 HT-29 cells labeling E-Cadherin Antibody (HY-P80113, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, ICC/IF, IHC-P, FC, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human E-Cadherin.AA range:580-630.