FGFR2 抗体 (YA439)

FGFR2 Antibody (YA439) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGFR2.

FGFR2 是一种酪氨酸蛋白激酶 (tyrosine-protein kinase)，作为成纤维细胞生长因子 (fibroblast growth factors) 的细胞表面受体，在细胞增殖、分化、迁移和凋亡的调控以及胚胎发育中起关键作用。它对胚胎模式形成、滋养层功能、肢芽发育、肺形态发生、骨生成和皮肤发育至关重要。FGFR2 通过磷酸化 PLCG1、FRS2 和 PAK4 等底物激活下游信号通路，包括 RAS-MAPK 和 AKT1 通路，但其信号活性受泛素化、内化和降解的负调控。功能获得性突变或成熟障碍会导致异常信号传导，而过表达则促进 STAT1 激活。by similar: 该蛋白在角质形成细胞和未成熟成骨细胞中促进增殖，但在分化成骨细胞中诱导凋亡。

Free

P21802

Predicted band size: 92 kDa;Observed band size: 145 kDa

Protein A affinity purified.

Cell membrane, Golgi apparatus, Cytoplasmic vesicle.

Non-conjugated

Unmodified

AB_3102482

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000-1:2000; IP: 1:10-1:50

• 
  Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using FGFR2 (HY-P80397) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80397, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human FGFR2.AA range:30-70.