HDAC1 抗体 (YA395)

HDAC1 Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to HDAC1. It can be used as a loading control antibody.

HDAC1 是一种组蛋白去乙酰化酶，催化核心组蛋白（H2A、H2B、H3 和 H4）N 端部分赖氨酸残基的去乙酰化。组蛋白去乙酰化为表观遗传抑制提供标记，并在转录调控、细胞周期进程和发育事件中发挥重要作用。HDAC1 通过形成大型多蛋白复合物发挥作用，作为组蛋白去乙酰化酶 NuRD 复合物的组成部分参与染色质重塑。作为 SIN3B 复合物的一部分，它通过组蛋白相互作用被招募到组成型活性基因转录起始位点的下游，并减轻组蛋白乙酰化和 RNA 聚合酶 II 在转录区域内的进程，从而调控转录。HDAC1 还可作为非组蛋白靶标（如 NR1D2、RELA、SP1、SP3、STAT3 和 TSHZ3）的去乙酰化酶。它去乙酰化 SP 蛋白 SP1 和 SP3 并调节其功能。此外，HDAC1 是 BRG1-RB1-HDAC1 复合物的组成部分，负调控静息神经元中 CREST 介导的转录。在钙刺激下，HDAC1 从复合物中释放，CREBBP 被招募，从而促进转录激活。HDAC1 还去乙酰化 TSHZ3 并调节其转录抑制活性，去乙酰化 RELA 中的 'Lys-310' 从而抑制 NF-kappa-B 的转录活性，去乙酰化 NR1D2 并消除 KAT5 介导的 NR1D2 转录抑制活性缓解效应。类似功能包括：作为 RCOR/GFI/KDM1A/HDAC 复合物的组成部分，通过组蛋白去乙酰化酶（HDAC）招募抑制多系血细胞发育相关基因；参与 CIART 介导的昼夜节律转录激活因子 CLOCK-BMAL1 异二聚体的转录抑制；通过组蛋白去乙酰化介导大型 PER 复合物或 CRY1 对昼夜节律靶基因（如 PER1）的转录抑制。此外，HDAC1 还具有蛋白质-赖氨酸去酰化酶活性：作为蛋白质去巴豆酰化酶和去乳酸酰化酶，分别介导组蛋白的去巴豆酰化（(2E)-丁烯酰）和去乳酸酰化（乳酸酰）。

Free

Q13547

Predicted band size: 55 kDa; Observed band size: 65 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102094

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2000; IHC-P: 1:50-1:800; ICC: 1:50

• 
  Western blot analysis of extracts from HEK293(lane2(20μg) , Hela(lane 3(20μg) and Jurkat(lane 4(20ug) using HDAC1 Antibody (HY-P80149) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling HDAC1with HDAC1 Antibody (HY-P80149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC1 Antibody (HY-P80149) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling HDAC1 with HDAC1 Antibody (HY-P80149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC1Antibody (HY-P80149)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80149, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80149, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC1.AA range:420-460.