HDAC4 抗体 (YA391)

HDAC4 Antibody (YA391) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC4.

HDAC4 蛋白在核心组蛋白（H2A、H2B、H3 和 H4）N 端区域赖氨酸残基的脱乙酰化中发挥着至关重要的作用，有助于表观遗传抑制，而表观遗传抑制是重要细胞过程的基础，包括转录调控、细胞周期进展和发展事件。组蛋白脱乙酰酶（包括 HDAC4）在大型多蛋白复合物中发挥作用，协调这些复杂的调节机制。具体来说，HDAC4 通过与肌细胞增强因子（MEF2A、MEF2C 和 MEF2D）相互作用参与肌肉成熟。此外，HDAC4 在 MTA1 介导的乳腺癌 ESR1 表达的表观遗传调控中发挥着关键作用。值得注意的是，HDAC4 在“Lys-77”处使 HSPA1A 和 HSPA1B 去乙酰化，导致它们优先与辅助伴侣 STUB1 结合，证明该蛋白质在细胞过程和分子相互作用中具有多样化和微妙的参与。

Free

P56524

Predicted band size: 119 kDa; Observed band size: 150 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102097

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000-1:2000; ICC/IF: 1:100-1:500

• 
  Western blot analysis of extracts from HCT116(lane 2(20μg) and HCT116(lane 3(40μg) using HDAC4(HY-P80153 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling HDAC4 with HDAC4 Antibody (HY-P80153) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC4 Antibody (HY-P80153)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling HDAC4 with HDAC4 Antibody (HY-P80153) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC4 Antibody (HY-P80153) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC4.AA range:11-125.