HDAC6 抗体 (YA390)

HDAC6 Antibody (YA390) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC6.

HDAC6 蛋白承担多方面的作用，主要负责核心组蛋白（H2A、H2B、H3 和 H4）N 端部分赖氨酸残基的脱乙酰化，有助于表观遗传抑制，在转录调控、细胞周期进展和发育事件。组蛋白脱乙酰酶（包括 HDAC6）在大型多蛋白复合物中运作，协调这些过程。除了组蛋白之外，HDAC6 还将其去乙酰化活性扩展到多种蛋白质，例如微管蛋白（对于微管依赖性细胞运动至关重要）和 α-微管蛋白（对于纤毛分解至关重要）。此外，HDAC6 通过 CTTN 去乙酰化促进肌动蛋白聚合和自噬体-溶酶体融合，促进自噬完成。它参与 MTA1 介导的乳腺癌 ESR1 表达的表观遗传调控和促进成牙本质细胞分化，展示了其多种功能。此外，HDAC6在错误折叠蛋白的降解中发挥着关键作用，当分子伴侣重折叠系统和泛素蛋白酶体不足时，HDAC6充当识别多泛素化错误折叠蛋白的接头，并将其靶向聚集体，通过自噬清除。

Free

Q9UBN7

Predicted band size: 131 kDa; Observed band size: 160 kDa

Protein A affinity purified.

Cell projection, Cytoplasm, Cytoskeleton, Nucleus.

Non-conjugated

Unmodified

AB_3102320

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000-1:5000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:400; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using HDAC6 Antibody (HY-P80154) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling HDAC6 Antibody (HY-P80154) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC6 Antibody (HY-P80154) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling HDAC6 Antibody (HY-P80154) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC6 Antibody (HY-P80154) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC6.AA range:1-50.