HtrA2/Omi 抗体 (YA726)

HtrA2/Omi Antibody (YA726) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HtrA2/Omi.

HtrA2 (8G11)：该基因编码一种丝氨酸蛋白酶。该蛋白定位于内质网，并与有丝分裂原活化蛋白激酶 14 的可变剪接形式相互作用。该蛋白还定位于线粒体，并在细胞凋亡刺激后释放到胞质溶胶中。该蛋白被认为通过结合细胞凋亡抑制蛋白杆状病毒 IAP 重复序列 4 来诱导细胞凋亡。还观察到了该蛋白的核定位。该基因的交替剪接导致编码不同亚型的多个转录变体。[由 RefSeq 提供，2016 年 3 月]

Free

O43464

Predicted band size: 49 kDa; Observed band size: 37 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102674

Neuroscience

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:1000; IP: 1:20

• 
  Western blot analysis of extracts from HEK293(lane 2(20ug) , Hela(lane 3(20ug) and Jurkat(lane 4(20ug) using HtrA2 Antibody (HY-P80715) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human HtrA2/Omi.The exact sequence is proprietary to MCE.