KMT6/EZH2 抗体 (YA320)

KMT6/EZH2 Antibody (YA320) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KMT6/EZH2.

EZH2 是一种多梳族 (PcG) 蛋白，作为 PRC2/EED-EZH2 复合物的催化亚基，有助于组蛋白甲基化和随后的转录抑制。该复合物可将组蛋白 H3 的“Lys-27”(H3K27) 进行单甲基化、二甲基化和三甲基化，分别生成 H3K27me1、H3K27me2 和 H3K27me3。EZH2 表现出对甲基化程度较低的底物的偏好，并且对于在胚胎干细胞中形成 H3K27me3 至关重要，在维持干细胞身份和促进正确分化方面发挥着至关重要的作用。此外，PRC2/EED-EZH2复合物可以作为招募DNA甲基转移酶的平台，在不同的表观遗传抑制系统之间建立联系。该复合物抑制的靶基因包括 HOXC8、HOXA9、MYT1、CDKN2A 和视黄酸反应基因。EZH2 将其甲基化活性从组蛋白扩展到非组蛋白，例如转录因子 GATA4 和核受体 RORA。此外，EZH2 通过昼夜节律基因启动子处的组蛋白甲基化来调节昼夜节律时钟，从而有助于 CRY1/2 介导的对 CLOCK-BMAL1 驱动的 PER1/2 转录激活的抑制。这种多方面的参与强调了 EZH2 在表观遗传调控和细胞过程中不可或缺的作用。

Free

Q15910

Predicted band size: 85 kDa; Observed band size: 98 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102580

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

ICC/IF: 1:50-1:200; IHC-P: 1:50-1:400

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(20μg) using Cleaved PARP(HY-P80507) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling KMT6/EZH2 with KMT6/EZH2 Antibody (HY-P80507) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KMT6/EZH2 Antibody (HY-P80507) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling KMT6/EZH2 with KMT6/EZH2 Antibody (HY-P80507) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KMT6/EZH2 Antibody (HY-P80507)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

IHC-P, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human EZH2.AA range:140-290.