MCM2 抗体 (YA705)

MCM2 Antibody (YA705) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCM2.

MCM2 (2B5)：由该基因编码的蛋白质是高度保守的微型染色体维持蛋白 (MCM) 之一，参与真核基因组复制的启动。由 MCM 蛋白形成的六聚体蛋白复合物是复制前复合物 (pre_RC) 的关键组成部分，可能参与复制叉的形成和其他 DNA 复制相关蛋白的募集。该蛋白质与 MCM4、6 和 7 形成复合物，并已显示可调节复合物的解旋酶活性。这种蛋白质被磷酸化，因此受蛋白激酶 CDC2 和 CDC7 的调节。已发现多个可变剪接的转录本变体，但尚未定义某些变体的全长性质。[由 RefSeq 提供，2012 年 10 月]

Free

P49736

Predicted band size: 102 kDa; Observed band size: 125 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102691

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Jurkat(lane 2(20ug) , K562(lane 3(20ug) and NIH/3T3(lane 4(20ug) using MCM2 Antibody (HY-P80749) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using MCM2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using MCM2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling MCM2 Antibody (YA705) (HY-P80749, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IHC-F, IHC-P, ICC/IF, FC, IP

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding tofragment of Human MCM2.The exact sequence is proprietary to MCE.