Mitofusin-2 抗体

Mitofusin-2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Mitofusin-2.

Mitofusin 2：该基因编码一种参与线粒体融合的线粒体膜蛋白，有助于线粒体网络的维持和运行。该蛋白参与血管平滑肌细胞增殖的调节，可能在肥胖的病理生理学中发挥作用。该基因的突变会导致 Charcot-Marie-Tooth 病 2A2 型和遗传性运动和感觉神经病 VI，这两种疾病都是周围神经系统疾病。该基因的缺陷也与早发性中风有关。已鉴定出编码相同蛋白质的两种转录变体。[由 RefSeq 提供，2008 年 7 月]

Free

O95140

Predicted band size: 86 kDa; Observed band size: 86 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102693

Tags & Cell Markers

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; ELISA: 1:10000

• 
  Western blot analysis of extracts from HUVEC (lane 2(20μg) and HUVEC (lane 3(20μg) using Mitofusin 2 (HY-P80752) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Mitofusin 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80752, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Mitofusin 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80752, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Mfn2.AA range:354-403.