MyD88 抗体 (YA280)

MyD88 Antibody (YA280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MyD88.

MYD88 蛋白作为衔接蛋白，在先天免疫反应中的 Toll 样受体 (TLR) 和 IL-1 受体信号通路中发挥着至关重要的作用。MYD88 通过 IRAK1、IRAK2、IRF7 和 TRAF6 发挥作用，触发 NF-kappa-B 激活、细胞因子分泌和炎症反应。此外，它还能增强 IL-8 转录并参与 IL-18 介导的信号通路。MYD88 激活 IRF1，促进其快速易位到细胞核中，从而有效诱导 IFN-β、NOS2/INOS 和 IL12A 基因。值得注意的是，来自 SARS-CoV-2、SARS-CoV 和 HIV-1 等病毒的富含 GU 的单链 RNA 激活 TLR8 后，MYD88 通过 NLRP3 炎性体激活诱导 IL1B 释放。在肠上皮细胞中，MYD88 介导的信号传导对于维持肠道稳态和调节小肠中抗菌凝集素 REG3G 的表达至关重要。MYD88 与 TIRAP 形成同二聚体和异二聚体，结合 TLR2、TLR4、TLR5、IRAK1、IRAK2、IRAK4，并与多种蛋白质相互作用，包括 IL18R1、BMX、IL1RL1、IKBKE、IRF7、LRRFIP1、LRRFIP2、FLII、IRF1、PELI1 和DHX9 等，从而编排涉及不同信号通路的分子相互作用的复杂网络。

Free

Q99836

Predicted band size: 33 kDa;Observed band size: 36 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3102366

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from K562(lane 2(20μg) or lane 3(40μg)) and Raji(lane 4(20μg) or lane 5(40μg)), using MyD88 (HY-P80233) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80233, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human MyD88.AA range:1-49.