PARP2 抗体 (YA244)

PARP2 Antibody (YA244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PARP2.

PARP2 是一种 poly-ADP-ribosyltransferase，介导蛋白质的 poly-ADP-ribosylation，在 DNA 修复中起关键作用。它能够催化谷氨酸（glutamate）、天冬氨酸（aspartate）或丝氨酸（serine）的 ADP-ribosylation，将 NAD(+) 的 ADP-D-ribosyl 基团转移到目标蛋白的羧基上，并进一步形成平均长度为 20-30 个单位的聚合物。在 DNA 损伤响应中，丝氨酸 ADP-ribosylation 是主要形式。在没有 HPF1 时，PARP2 介导谷氨酸和天冬氨酸的 ADP-ribosylation；而与 HPF1 结合后，则特异性催化丝氨酸 ADP-ribosylation。PARP2 通过识别染色质中的 DNA 断裂并招募 HPF1，促进组蛋白等靶蛋白的丝氨酸 ADP-ribosylation，从而松弛染色质结构并招募修复因子，修复 DNA 链断裂。HPF1 会限制 PARP2 的聚合酶活性以控制 poly-ADP-ribose 链的长度。此外，PARP2 还能特异性介导分支型 poly-ADP-ribosylation，并被 APLF 等因子识别。除了蛋白质，PARP2 还能 ADP-ribosylate DNA，优先作用于 nicked duplex 中 5'-末端磷酸基团。

Free

Q9UGN5

Predicted band size: 66 kDa; Observed band size: 66 kDa

Protein A affinity purified.

Chromosome, Nucleus.

Non-conjugated

Unmodified

AB_3102389

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and Raji(lane 4(20μg) or lane 5(40μg)), using PARP2 (HY-P80265) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80265, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PARP2.AA range:484-583.