1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. PDX1 抗体 (YA234)

PDX1 抗体 (YA234)

目录号: HY-P80274
COA 抗体使用指南 技术支持

PDX1 Antibody (YA234) 是一个兔来源、无偶联标记、抗 PDX1 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

PDX1 Antibody (YA234) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDX1.

研究背景

PDX1 是一种转录因子,能够激活 insulin、somatostatin、glucokinase、islet amyloid polypeptide 和 glucose transporter type 2 基因的转录,尤其在葡萄糖依赖性胰岛素基因转录调控中发挥关键作用。在胰腺腺泡细胞中,PDX1 作为 PDX1:PBX1b:MEIS2b 复合物的一部分,参与 ELA1 增强子的转录激活;该复合物结合于增强子 B 元件,并与结合在增强子 A 元件上的转录因子 1 复合物 (PTF1) 协同作用。PDX1 优先结合 DNA 基序 5'-[CT]TAAT[TG]-3'。在发育过程中,PDX1 决定早期胰腺上皮细胞的特性,促进其增殖、分支及后续分化。在成年阶段,PDX1 对维持 β 细胞激素分泌表型至关重要。

标签

Free

基因 ID
蛋白数据库
中文名
PDX1 抗体
分子量

Predicted band size: 31 kDa;Observed band size: 45 kDa

纯度

Protein A affinity purified.

亚细胞定位

Cytosol, Nucleus.

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Signal Transduction

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human

推荐稀释比例

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:800 ;ICC/IF: 1:50-1:200

  • Western blot analysis of extracts from Hela(lane 2(20μg) and Hela(lane 3(40μg) using PDX1(HY-P80274 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Immunocytochemistry analysis of PANC-1 cells labeling PDX1 with PDX1 Antibody (HY-P80274) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PDX1 Antibody (HY-P80274) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Immunocytochemistry analysis of Hela cells labeling PDX1 with PDX1Antibody (HY-P80274) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PDX1 Antibody (HY-P80274)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
应用

WB, IHC-P, ICC/IF

性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

Synthetic peptide corresponding to Human PDX1.AA range:234-283.

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
PDX1 Antibody (YA234)
目录号:
HY-P80274
需求量: