Phospho-CDK1/2/3 (Thr14) 抗体 (YA218)

Phospho-CDK1/2/3 (Thr14) Antibody (YA218) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-CDK1/2/3 (Thr14).

CDC2 是一种关键蛋白，通过调节中心体周期和有丝分裂起始在真核细胞周期调控中发挥核心作用。它通过与多种间期 cyclins 结合促进 G2-M 期转换，并磷酸化包括 PARVA/actopaxin、APC、BARD1 等在内的众多底物，参与中心体分离、高尔基体动态、核膜破裂和染色体凝聚等过程。CDC2 还调控纺锤体形成、微管动力学及细胞凋亡相关信号（如磷酸化 Bcl-xL/BCL2L1 触发凋亡，而磷酸化 CASP8 则抑制其活化）。在 DNA 损伤时，CDC2 活性被抑制以实现 G2 期检查点阻滞；修复后通过 WIP1 信号重新激活。此外，CDC2 磷酸化核心时钟基因调控因子 NR1D1 影响生物钟振幅，并在有丝分裂中抑制 CGAS 以防止自身 DNA 激活免疫应答。类似功能包括磷酸化 KRT5（by similar）和 CALD1（by similar）以分别参与中期进程和雪旺细胞迁移。CDC2 还可作为丙型肝炎病毒（HCV）进入肝细胞的受体。

Free

P06493 / P24941 / Q00526

Predicted band size: 34 kDa; Observed band size: 34 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102195

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Hamster

WB: 1:500-1:1000; IP: 1:20

• 
  Western blot analysis of extracts from SiHa(lane 2(20ug) , C2C12(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Phospho-CDK1/2/3 Antibody (HY-P80797) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr14 of Human Cdk1.The exact sequence is proprietary to MCE.