Phospho-Src (Tyr419) 抗体 (YA150)

Phospho-Src (Tyr419) Antibody (YA150) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Src (Tyr419).

Src 是一种非受体蛋白酪氨酸激酶，在多种受体（包括免疫反应受体、整合素、受体蛋白酪氨酸激酶、G 蛋白偶联受体和细胞因子受体）的参与触发的一系列细胞过程中发挥着核心作用。。它协调控制基因转录、免疫反应、细胞粘附、细胞周期进程、细胞凋亡、迁移和细胞转化的信号通路。虽然 SRC 激酶家族内的功能冗余使特定作用的识别变得复杂，但 SRC 似乎是受体结合后激活的主要激酶，影响其他蛋白酪氨酸激酶 (PTK) 家族的激活。SRC 通过磷酸化 AFAP1 和 cortactin (CTTN) 等底物参与细胞骨架组织的调节。它的参与延伸到细胞-细胞和细胞-基质连接，在那里它磷酸化β-连环蛋白 (CTNNB1)、δ-连环蛋白 (CTNND1)、斑球蛋白 (JUP) 和连接蛋白-43 (GJA1) 等成分。此外，SRC 参与 PDGF 介导的 STAT1 和 STAT3 酪氨酸磷酸化、RAS 途径、EGF 介导的钙激活氯离子通道激活和 β-抑制蛋白脱敏。它还在 EGFR 内化、CDK20/MAPK3 丝裂原激活蛋白激酶级联刺激和破骨细胞骨吸收中发挥着至关重要的作用。SRC 的多方面活动凸显了其在细胞调节和信号转导中的复杂作用。

Free

P12931

Predicted band size: 60 kDa; Observed band size: 60 kDa

Protein A affinity purified.

Cell membrane, Mitochondrion inner membrane, Nucleus, Cytoplasm.

Non-conjugated

Phosphorylated

AB_3102587

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(20μg), Calyculin A treated (HY-18983, 100ng/mL, 2hrs) as indicated using Phospho-Src (Tyr419) (HY-P80514) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-Src (Tyr419) Antibody(HY-P80514, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr419 of Human Src.AA range:401-450.