2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体 流式抗体
  5. RAB7 抗体 (YA110)

RAB7 Antibody (YA110) 是一个兔来源、无偶联标记、抗 RAB7 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥493 In-stock
50 μL ￥1281 In-stock
100 μL ￥2100 In-stock
250 μL   询价  

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

RAB7 Antibody (YA110) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAB7.
宿主

Rabbit
克隆性

Recombinant, Monoclonal
分子量
Predicted band size: 23 kDa;
Observed band size: 23 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human RAB7.AA range:158-207.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:2000
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-1:500
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
FC
FC: 流式细胞术
1:50-1:100
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL ICC WB FC

  • Immunocytochemistry analysis of NIH3T3 cells labeling RAB7 with RAB7 antibody (HY-P80295) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RAB7 antibody (HY-P80295) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of NIH3T3 cells labeling RAB7 with RAB7 antibody (HY-P80295) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RAB7 antibody (HY-P80295) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Western blot analysis of extracts from Hela (lane 2(20μg), C2C12 (lane 3(20μg), C6 (lane 4(20μg) using CD44 (HY-P80295) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80295, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Flow cytometric analysis of 1X10^6 K562 cells labeling RAB7 Antibody (HY-P80295, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

背景
功能：小 GTP 酶 Rab 是细胞内膜运输的关键调控因子，其作用涵盖从运输囊泡的形成到囊泡与膜的融合。Rab 蛋白在非活性 GDP 结合态和活性 GTP 结合态之间循环，后者能够募集不同的下游效应蛋白至膜上，这些效应蛋白直接负责囊泡的形成、运动、系留和融合 (PubMed:38538795)。在活性状态下，RAB7A 与多种效应蛋白结合，在内体-溶酶体运输的调控中发挥关键作用。它调控内体从早期到晚期的成熟过程、微管负端和正端引导的内体迁移和定位，以及通过不同的蛋白质-蛋白质相互作用级联反应进行的内体-溶酶体运输。此外，RAB7A 在生长因子介导的细胞信号传导、营养转运蛋白介导的营养吸收、神经元轴突中的神经营养因子运输以及脂质代谢中也发挥着核心作用。它还参与调控一些特殊的内体膜运输，例如黑素体的成熟、病原体诱导的吞噬体 (或液泡) 和自噬体。它在吞噬病原体 (例如金黄色葡萄球菌和结核分枝杆菌) 的吞噬体的成熟和酸化过程中发挥作用。它还参与吞噬体与溶酶体的融合。与 RAC1 协同作用，它参与调控破骨细胞中皱褶缘 (RB) 的形成。它控制 NTRK1/TRKA 的内体运输和神经突生长信号传导 (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211)。通过调控溶酶体降解来调节 EGF-EGFR 复合物的内吞转运。参与 ADRB2 刺激的脂解作用，脂解作用是一种胞质脂肪酶非依赖性自噬途径 (基于相似性)。是 SDCBP、CD63 和 syndecan 外泌体释放所必需的 (PubMed:22660413)。是 ACE2 囊泡运输和细胞表面表达所必需的 (PubMed:33147445)。可能在 PRPH 神经元中间丝组装中发挥作用 (基于相似性)。
亚细胞定位：细胞质囊泡，吞噬体膜；外周膜蛋白；细胞质侧；晚期内体膜；外周膜蛋白；细胞质侧；溶酶体膜；外周膜蛋白；细胞质侧；黑素体膜；外周膜蛋白；细胞质侧；细胞质囊泡，自噬体膜；外周膜蛋白；细胞质侧；脂滴；内体膜；外周膜蛋白；细胞质囊泡；线粒体膜；外周膜蛋白
表达水平：
组织特异性：广泛表达；在骨骼肌中高表达
亚基：GTP 结合形式与 RAC1 相互作用 (基于相似性)。与 NTRK1/TRKA 相互作用 (基于相似性)。与 C9orf72 相互作用 (基于相似性)。与 Rab 牻牛儿基牻牛儿基转移酶复合物的底物结合亚基 CHM 相互作用 (基于相似性)。与 RILP 相互作用 (PubMed:11179213, PubMed:14668488, PubMed:15933719, PubMed:20028791, PubMed:22431521)。与 PSMA7 相互作用 (PubMed:14998988)。与 RNF115 相互作用 (PubMed:16925951)。与 FYCO1 相互作用 (PubMed:20100911)。与 PIK3C3/VPS34-PIK3R4 复合物相互作用 (PubMed:14617358)。GTP 结合形式与 OSBPL1A 相互作用 (PubMed:16176980)。与 CLN3 相互作用 (PubMed:22261744)。不与 HPS4 和 BLOC-3 复合物 (HPS1 和 HPS4 的异二聚体) 相互作用 (PubMed:20048159)。与 CLN5 相互作用 (PubMed:22431521)。与 PLEKHM1 相互作用 (通过 N 端和 C 端)(PubMed:28325809)。与 PRPH 相互作用；该相互作用是直接的 (PubMed:23179371)。与 VPS13A 相互作用 (PubMed:30709847)。 GDP 结合形式与 RIMOC1 相互作用 (PubMed:34432599)。与 MON1A-CCZ1B 复合物相互作用，且在 RIMOC1 存在下该相互作用增强 (PubMed:34432599)。与 VPS39 和 VPS41 相互作用 (PubMed:37821429)。与 RUFY4 相互作用 (PubMed:26416964)。与 LAMP2 和 RUFY4 形成三元复合物；与 LAMP2 的相互作用由 RUFY4 介导 (通过 RUN 结构域和卷曲螺旋结构域)(基于相似性)。
RRID
反应种属数据库

Entrez Gene: 7879 Human ; 19349 Mouse ;

SwissProt: P51149 Human ; P51150 Mouse ;

OMIM: 600882 Human

研究领域

Tags & Cell Markers
中文名
RAB7 抗体
同用名
CMT2B antibody; PRO2706 antibody; PSN antibody; RAB7, member RAS oncogene family antibody; RAB7A antibody; RAB7A, member RAS oncogene family antibody; RAB7A_HUMAN antibody; Ras associated protein RAB7 antibody; Ras related protein Rab7 antibody; Ras related protein Rab7a antibody
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

RAB7 Antibody (YA110) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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