2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体
  5. RelB 抗体 (YA098)

RelB Antibody (YA098) 是一个兔来源、无偶联标记、抗 RelB 的 IgG 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μL ￥493 In-stock
50 μL ￥1281 In-stock
100 μL ￥2100 In-stock
250 μL   询价  

* Please select Quantity before adding items.

实现从蛋白分离到检测的高效衔接！

加购更优惠，一站式完成电泳实验！
Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

RelB Antibody (YA098) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RelB.
宿主

Rabbit
克隆性

Recombinant, Monoclonal
分子量
Predicted band size: 62 kDa;
Observed band size: 70 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human RelB.AA range:1-50.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:1000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
IP
IP: 免疫沉淀
Use at an assay dependent concentration.
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL WB ICC

  • Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

背景
功能：NF-κB 是一种多效性转录因子，几乎存在于所有细胞类型中，参与多种生物学过程，例如炎症、免疫、分化、细胞生长、肿瘤发生和细胞凋亡。NF-κB 是由含有 Rel 样结构域的蛋白 RELA/p65、RELB、NFKB1/p105、NFKB1/p50、REL 和 NFKB2/p52 组成的同源或异源二聚体复合物。这些二聚体结合于靶基因 DNA 上的κB 结合位点，不同的二聚体对不同的κB 结合位点具有不同的偏好性，能够以不同的亲和力和特异性结合。不同的二聚体组合分别作为转录激活因子或抑制因子发挥作用。NF-κB 的活性受多种机制调控，包括翻译后修饰、亚细胞区室化以及与其他辅助因子或共抑制因子的相互作用。 NF-κB 复合物以非活性状态存在于细胞质中，并与 NF-κB 抑制剂 (I-κB) 家族成员结合。在传统的激活途径中，I-κB 在不同激活因子的刺激下被 I-κB 激酶 (IKK) 磷酸化，随后被降解，从而释放出活性 NF-κB 复合物并转位至细胞核。NF-κB 异二聚体 RelB-p50 和 RelB-p52 复合物是转录激活因子。RELB 既不与 DNA 结合，也不与 RELA/p65 或 REL 结合。在 NFKB2/p49 存在的情况下，RELB 可刺激启动子活性。作为 NUPR1/RELB/IER3 生存通路的一员，RELB 可能赋予胰腺导管腺癌对细胞应激 (例如饥饿或吉西他滨治疗) 的显著抵抗力。通过抑制 CLOCK-BMAL1 异二聚体的转录激活活性来调节生物钟，且该抑制作用不依赖于 CRY1/CRY2。在 NFKB2/p52 存在的情况下，该异二聚体的抑制作用增强。该蛋白对 T 细胞和 B 细胞的成熟和功能均是必需的 (PubMed:26385063)。
亚细胞定位：细胞核；细胞质、细胞骨架、微管组织中心、中心体
表达水平：
诱导：受促分裂原和NUPR1上调
亚基：NF-κB RelB-p50 复合物的组成部分。NF-κB RelB-p52 复合物的组成部分。可自缔合；这种相互作用似乎是短暂的，可能阻止降解，从而与 p50 或 p52 形成异二聚体。与 NFKB1/p50、NFKB2/p52 和 NFKB2/p100 相互作用。与 NFKBID 相互作用。与 BMAL1 相互作用，且在 CLOCK 存在下 (基于相似性) 这种相互作用增强。
RRID
反应种属数据库

Entrez Gene: 5971 Human

SwissProt: Q01201 Human

OMIM: 617585 Human

研究领域

Cell Biology
中文名
RelB 抗体
文件资料

COA (194 KB)

抗体使用指南 (1083 KB)

RelB Antibody (YA098) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
RelB Antibody (YA098)
目录号：
HY-P80306
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z