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  2. 一抗
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  4. RUNX2 抗体 (YA085)

RUNX2 抗体 (YA085)

目录号: HY-P80316
COA 抗体使用指南 技术支持

RUNX2 Antibody (YA085) 是一个兔来源、无偶联标记、抗 RUNX2IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

RUNX2 Antibody (YA085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RUNX2.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 57 kDa;
Observed band size: 57 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human RUNX2.AA range:300-450.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:500
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from NIH3T3 (lane 2(20μg) , MDA-MB231(lane 3(20μg) and Saos-2(lane 4(20μg) using RUNX2(HY-P80316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:参与成骨细胞分化和骨骼形态发生的转录因子 (PubMed:28505335, PubMed:28703881, PubMed:28738062)。对成骨细胞的成熟以及膜内成骨和软骨内成骨均至关重要。CBF 与多种增强子和启动子的核心位点 5'-PYGPYGGT-3' 结合,包括鼠白血病病毒、多瘤病毒增强子、T 细胞受体增强子、骨钙素、骨桥蛋白、骨唾液蛋白、α1 (I) 型胶原、LCK、IL-3 和 GM-CSF 启动子。在成骨细胞中,CBF 支持转录激活:与 SPEN/MINT 协同作用,增强 FGFR2 介导的骨钙素 FGF 反应元件 (OCFRE) 的激活 (基于相似性)。抑制 KAT6B 依赖性转录激活
亚细胞定位:细胞核;细胞质
表达水平:
组织特异性:特异性表达于成骨细胞
亚基:由 α 亚基和 β 亚基组成的异二聚体。 α 亚基以单体形式以及通过 Runt 结构域结合 DNA。异二聚化可增强 DNA 结合能力。与 XRCC6 (Ku70) 和 XRCC5 (Ku80) 相互作用。与 HIVEP3 相互作用。与 IFI204 相互作用。与 SATB2 相互作用;该相互作用增强了这些转录因子对 DNA 的结合和转录激活作用。与 HIPK3 结合。与 FOXO1 (通过 C 端区域) 相互作用;该相互作用抑制 RUNX2 对 BGLAP 的转录活性。胰岛素或 IGF1 刺激可阻止这种相互作用,因为 FOXO1 会从细胞核输出 (基于相似性)。与 CCNB1、KAT6A 和 KAT6B 相互作用。与 FOXP3 相互作用。与 TMEM119 相互作用 (基于相似性)。与 OLFM2 相互作用 (基于相似性)。与 IPO7 相互作用;该相互作用抑制成骨细胞中 RUNX2 的核转位 (基于相似性)。
RRID
反应种属数据库
研究领域

Epigenetics and Nuclear Signaling

中文名
RUNX2 抗体
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RUNX2 Antibody (YA085)
目录号:
HY-P80316
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