STING 抗体 (YA048)

STING Antibody (YA048) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STING.

TMEM173 蛋白充当先天免疫信号传导的促进剂，充当细菌和病毒胞质 DNA 的传感器，最终促进 I 型干扰素（IFN-α 和 IFN-β）的产生。这种先天免疫反应是对来自病毒和细菌的非 CpG 双链 DNA 传递到细胞质的反应而触发的。TMEM173 识别并结合环状二核苷酸，特别是环状二 GMP (c-di-GMP)（细菌产生的第二信使）和环状 GMP-AMP (cGAMP)（CGAS 响应细胞质中的 DNA 病毒而产生的信使）。与 c-di-GMP 或 cGAMP 结合后，TMEM173 寡聚化，从内质网移位，并被 TBK1 磷酸化，导致转录因子 IRF3 的募集和激活。这会诱导 I 型干扰素的表达，建立有效的抗病毒状态。此外，TMEM173 在自噬中发挥直接作用，cGAMP 结合启动 ERGIC 形成，ERGIC 作为自噬体形成的膜源，靶向胞质 DNA 或 DNA 病毒进行溶酶体降解。TMEM173 的多方面活性凸显了其在协调先天免疫反应和自噬过程中的核心作用，有助于细胞防御病毒和细菌威胁。

Free

Q86WV6

Predicted band size: 42 kDa; Observed band size: 42 kDa

Protein A affinity purified.

Endoplasmic reticulum membrane, Cytoplasm, perinuclear region, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Mitochondrion outer membrane, Cell membrane.

Non-conjugated

Unmodified

AB_3102448

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from THP-1 (lane 2(20μg) , THP-1 (lane 3(40μg),using STING Antibody (HY-P80350). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Flow cytometric analysis of 1X10^6 THP-1 cells labeling STING Antibody (HY-P80350, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human TMEM173.AA range:220-379.