YAP1 抗体

YAP1 Antibody is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YAP1.

YAP1 是一种多方面的转录调节因子，是 Hippo 信号通路中的关键下游靶标，通过调节增殖和凋亡来协调器官大小控制和肿瘤抑制。该通路涉及激酶级联反应，其中 STK3/MST2 和 STK4/MST1 与 SAV1 协调，磷酸化并激活 LATS1/2，导致 YAP1 和 WWTR1/TAZ 癌蛋白磷酸化和失活。YAP1 通过 ARHGAP18 影响皮质肌动球蛋白网络的形成，在调节组织张力和 3D 组织形状方面发挥关键作用。此外，它在控制细胞增殖中发挥着关键作用，其核转位受到 LATS1/2 磷酸化的抑制。YAP1 与 TEAD 转录因子的协作对于刺激基因表达、细胞生长、贴壁独立生长和上皮间质转化 (EMT) 诱导至关重要。此外，它通过充当 TEAD4 靶基因 AURKA 和 PLK1 的转录辅阻遏物来抑制纤毛发生，并与 WWTR1 结合，调节 TGFB1 依赖性 SMAD2 和 SMAD3 核积累。YAP1 进一步激活 ERBB4 同工型 3 的 C 末端片段 (CTF)。

Free

P46937

Predicted band size: 54 kDa；Observed band size: 75 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102463

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), HepG2 (lane 3(20μg) and SiHa (lane 4(20μg) using YAP1 (HY-P80374) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using YAP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80374, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using YAP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80374, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling YAP1 Antibody(HY-P80374, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, ICC/IF, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human YAP1.AA range:421-470.