2. NF-κB Immunology/Inflammation Apoptosis GPCR/G Protein Cell Cycle/DNA Damage Stem Cell/Wnt
  3. NF-κB COX Interleukin Related TNF Receptor NO Synthase Prostaglandin Receptor CDK β-catenin Wnt Apoptosis
  4. Chikusetsusaponin IVa methyl ester

Chikusetsusaponin IVa methyl ester  (Synonyms: 竹节参皂苷IVA-6'-甲酯; CME)

目录号: HY-N17736
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Chikusetsusaponin IVa methyl ester (CME) 是一种三萜皂苷类天然化合物。Chikusetsusaponin IVa methyl ester 可通过抑制 Wnt/β-catenin 信号通路，诱导结肠癌细胞的 G0/G1 期细胞周期停滞和凋亡 (apoptosis)。Chikusetsusaponin IVa methyl ester 通过抑制 NF-κB 和 AP-1 信号通路，显著降低 NO、PGE₂ 以及促炎细胞因子 (TNF-α、IL-6、IL-1β) 的生成，下调 iNOS 和 COX-2 的水平。Chikusetsusaponin IVa methyl ester 可用于直肠癌和炎症研究。

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Chikusetsusaponin IVa methyl ester

Chikusetsusaponin IVa methyl ester Chemical Structure

CAS No. : 58546-61-5

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Chikusetsusaponin IVa methyl ester 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Chikusetsusaponin IVa methyl ester (CME) is a natural triterpenoid saponin compound. Chikusetsusaponin IVa methyl ester induces G0/G1 cell cycle arrest and apoptosis in colon cancer cells by inhibiting the Wnt/β-catenin signaling pathway. By inhibiting the NF-κB and AP-1 signaling pathways, Chikusetsusaponin IVa methyl ester significantly reduces the production of NO, PGE₂ and pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), and downregulates the levels of iNOS and COX-2. Chikusetsusaponin IVa methyl ester can be used in researches on colorectal cancer and inflammation[1][2].

体外研究
(In Vitro)

Chikusetsusaponin IVa methyl ester (3-30 μM；7-25 h) 可通过抑制 iNOS、COX-2、TNF-α、IL-6 和 IL-1β 的表达，以浓度依赖的方式抑制 LPS 诱导的 RAW264.7 巨噬细胞中促炎介质的产生及相关基因的表达[1]。
Chikusetsusaponin IVa methyl ester (3-30 μM；1.2-19 h) 可通过抑制 NF-κB p65 核转位及 c-Fos 的表达/磷酸化，以浓度依赖的方式抑制 RAW264.7 巨噬细胞中 LPS 诱导的 NF-κB 和 AP-1 活化[1]。
Chikusetsusaponin IVa methyl ester (12.5-100 μM；作用 24 h) 可抑制 HCT116 细胞的增殖，在 25 μM 浓度下会降低细胞活力，浓度高于 50 μM 时则会引发严重的细胞死亡[2]。
Chikusetsusaponin IVa methyl ester (10-40 μM) 可诱导 HCT116 细胞发生 G0/G1 期细胞周期阻滞[2]。
Chikusetsusaponin IVa methyl ester (20-30 μM；24 小时) 可在体外以时间和剂量依赖的方式下调 HCT116 细胞中的 cyclin D1、CDK2 和 CDK4，并上调 p21[2]。
Chikusetsusaponin IVa methyl ester (20-30 μM；作用 24 小时) 可呈剂量依赖性降低 HCT116 细胞中 β-catenin 与 TBE 的结合作用[2]。
Chikusetsusaponin IVa methyl ester (20-30 μM；处理 24 小时) 以剂量依赖的方式降低 HCT116 细胞中的核 β-catenin 水平 [2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Chikusetsusaponin IVa methyl ester 相关抗体:

RT-PCR[1]

Cell Line: RAW264.7
Concentration: 3 μM, 15 μM, 30 μM
Incubation Time: Pre-treatment for 1 h, followed by LPS stimulation for 6 h
Result: Concentration-dependently inhibited the mRNA expression of iNOS, COX-2, TNF-α, IL-6 and IL-1β.

Western Blot Analysis[1]

Cell Line: RAW264.7
Concentration: 3 μM, 15 μM, 30 μM
Incubation Time: Pre-treatment for 1 h, followed by LPS stimulation for 24 h
Result: Concentration-dependently inhibited the protein expression of iNOS, COX-2, TNF-α, IL-6 and IL-1β.

Cell Viability Assay[2]

Cell Line: HCT116 colon cancer cells
Concentration: 12.5, 25, 50 and 100 μM
Incubation Time: 24 h
Result: Decreased cell proliferation at 25 μM and 50 μM; caused severe cell death at concentrations above 50 μM.

Western Blot Analysis[2]

Cell Line: HCT116 colon cancer cells
Concentration: 20, 30 μM
Incubation Time: 4 h, 8 h, 24 h
Result: Decreased cyclin D1, CDK2, and CDK4 expression in a time- and dose-dependent manner; increased p21 expression.

Immunofluorescence[2]

Cell Line: HCT116 colon cancer cells
Concentration: 30 μM
Incubation Time: 0 h, 6 h, 24 h
Result: Suppressed the nuclear translocation of β-catenin over time.

Western Blot Analysis[2]

Cell Line: HCT116 colon cancer cells
Concentration: 20, 30 μM
Incubation Time: 24 h
Result: Decreased the level of β-catenin in the nucleus in a dose-dependent manner.

Real Time qPCR[2]

Cell Line: HCT116 colon cancer cells
Concentration: 20, 30 μM
Incubation Time: 24 h
Result: Decreased the mRNA expression of cyclin D1 and c-myc.
分子量

808.99

Formula

C43H68O14
CAS 号
中文名称

竹节参皂苷IVA-6'-甲酯
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Chikusetsusaponin IVa methyl ester 相关分类

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Keywords:

Chikusetsusaponin IVa methyl ester58546-61-5CME竹节参皂苷IVA-6'-甲酯NF-κBCOXInterleukin RelatedTNF ReceptorNO SynthaseProstaglandin ReceptorCDKβ-cateninWntApoptosisNuclear factor-κBNuclear factor-kappaBCyclooxygenaseILTumor Necrosis Factor ReceptorTNFRNitric oxide synthasesNOSCyclin dependent kinaseBeta cateninnitric oxide (NO)macrophagesp65 subunitinterleukin-6 (IL-6)Nuclear factor-kappaB (NF-κB)prostaglandin E2 (PGE2)tumor necrosis factor-alpha (TNF-α)c-FosActivator protein-1 (AP-1)Inhibitorinhibitorinhibit

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