2. Antibody-drug Conjugate/ADC Related Immunology/Inflammation Cell Cycle/DNA Damage Cytoskeleton
  3. Antibody-Drug Conjugates (ADCs) Transmembrane Glycoprotein Microtubule/Tubulin
  4. GLK-33

GLK-33 是一种靶向 CD33+ 的抗体药物偶联物 (ADC)。GLK-33 能与人源 CD33 及 CD33-Fc 融合蛋白结合，EC 50 介于 200 至 400 pM 之间。GLK-33 包含人源化抗 CD33 抗体 Lintuzumab (HY-P9983)，与药物-连接子偶联物 mavg-MMAU (HY-185154) 构成。GLK-33 表现出靶向特异性的抗体依赖性细胞毒性活性。GLK-33 能够降低 CD33 阳性白血病细胞及多药耐药细胞的活力和补体依赖性细胞毒作用 (CDC) 活性。GLK-33 在体内显示出抗肿瘤活性。GLK-33 可用于急性髓系白血病 (AML) 的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

GLK-33

GLK-33 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
1 mg   询价  
5 mg   询价  
10 mg   询价  

* Please select Quantity before adding items.

Customer Review

GLK-33 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GLK-33 is a CD33+ antibody-drug conjugate (ADC). GLK-33 binds to human CD33 with an EC 50 value between 200 and 400 pM. GLK-33 consists of the humanized anti-CD33 antibody Lintuzumab (HY-P9983), and the drug-linker conjugate being mavg-MMAU (HY-185154). GLK-33 shows target-specific antibody-dependent cellular cytotoxicity (ADCC) activity and complement-dependent cytotoxicity (CDC) activity against CD33-positive leukemia cell lines. GLK-33 reduces cell viability within CD33-positive leukemia and multidrug-resistant cells. GLK-33 demonstrates antitumor activity in vivo. GLK-33 can be used for acute myeloid leukemia (AML) research[1].
反应种属

Human
IC50 & Target

Siglec-3/CD33
体外研究
(In Vitro)

GLK-33 (0-24小时) 可结合并被 CD33+ 白血病细胞内化[1]。
GLK-33 不结合猴或小鼠的CD33-Fc融合蛋白[1]。
GLK-33 (1-10000 ng/mL，3-5 天) 对 CD33+ 白血病细胞系及多重耐药细胞系显示出靶向特异性的细胞毒活性[1]。
GLK-33 (0.1-10000 nM，30 分钟) 在 MOLM-13 与 NK 细胞共培养体系中显示出靶向特异性的 ADCC 活性，其 EC50 为5 nM[1]。
GLK-33 (1-10 µg/mL，0-24 小时) 在 HL-60 细胞中保留了 Fc 受体依赖性的 ADCC 及 CDC 免疫效应功能[1]。
GLK-33 (0.001-1000 nM，3-5天) 与 HL-60 细胞表面 CD33 的结合高度稳定，且不易受可溶性靶抗原的干扰[1]。
GLK-33 (5 天) 能有效靶向患者单个核细胞中的原始细胞，中位 IC50 值为 16 nM，且无脱靶淋巴细胞毒性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GLK-33 相关抗体:

Cell Viability Assay[1]

Cell Line: MV4-11, MOLM-13, HL-60, KG-1, OCI-M1, K-562, Daudi and Ramos cells
Concentration: 1, 10, 100, 1000 and 10000 ng/mL
Incubation Time: 3-5 days
Result: Exerted cytotoxicity against MV4-11, MOLM-13, HL-60, KG-1, OCI-M1 and K-562 cells with IC50 values of 0.14, 0.21, 0.24, 1.4, 2.7 and 725 nM, respectively.
Had no cytotoxicity to CD33-negative Daudi lymphoma cells (including Daudi and Ramos cells).
Inhibited KG-1 cells 3-fold more effectively in the presence of Verapamil (HY-14275).
体内研究
(In Vivo)

GLK-33 (300 µg/kg-3 mg/kg，单次静脉注射) 在皮下 HL-60 异种移植瘤小鼠模型中显示出高效的抗白血病活性[1]。
GLK-33 (100 µg/kg-1 mg/kg，单次静脉注射) 在皮下 MOLM-13 异种移植瘤中显示出靶向特异性的抗白血病肿瘤活性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HL-60 cells (2 x 104, s.c.) induced-Rj:ATHYMIC-Foxn1 nu/nu female mice (8 weeks, 17-23g)[1]
Dosage: 300 µg/kg, 1 mg/kg, 3 mg/kg
Administration: i.v., once
Result: Led to the disappearance of HL-60 xenograft tumors in all mice (100% complete responses, CR) at 3 mg/kg.
Removed the tumors in all mice (100% CR) at 1 mg/kg.
Significantly inhibited tumor growth at 300 µg/kg.
Animal Model: MOLM-13 cells (2 x 104, s.c.) induced-Rj:ATHYMIC-Foxn1nu/nu female mice (8 weeks, 17-23g)[1]
Dosage: 100 µg/kg, 300 µg/kg, 1 mg/kg, 3 mg/kg
Administration: i.v., once
Result: Eradicated the tumors in all mice (100% CR).
Showed dose-dependent activity at 100 µg/kg, 300 µg/kg, 1 mg/kg.
Inhibited tumor growth down to 300 mg/kg.
基因 ID

945  [NCBI]

Accession
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GLK-33 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GLK-33GLK33GLK 33Antibody-Drug Conjugates (ADCs)Transmembrane GlycoproteinMicrotubule/TubulinAntibody-Drug ConjugatesHumanizedADCCantitumor activityAcute Myeloid LeukemiaMDR+ cell linesCD33+ cellInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
GLK-33
目录号：
HY-P991910
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z