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  5. siRNA/miRNA Transfection Reagent

siRNA/miRNA Transfection Reagent 

siRNA/miRNA转染试剂

Cat. No.: HY-K2017
Manual COA SDS 技术支持

MCE siRNA/miRNA Transfection Reagent 是一种阳离子高分子聚合物的新型转染试剂,适用于 siRNA 和 miRNA 的转染。

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (50 μL)   Apply now  
1 mL ¥3600 In-stock
5 mL ¥12600 In-stock

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    MedChemExpress Validation
    Experimental conditions:
    Cell density: 40% density during cell transfection
    Dosage: 1 μL siRNA (EGFP/NC, 20 μM) + 2 μL transfection reagent (HY-K2017)
    Detection scheme: 36 h after siRNA transfection, use fluorescence microscopy to detect the interference effect of EGFP green fluorescent protein
    siRNA/miRNA Transfection Reagent (HY-K2017) demonstrated ​​high siRNA transfection efficiency and significant EGFP silencing in both A549 and HT29 cells.​

    MedChemExpress Validation
    Experimental Conditions:
    Cell Density: 40% of the cell density at the time of transfection
    Dosage: 2 μL siRNA (MALT1/NC, 20 μM) + 4 μL transfection reagent
    Assay: 36 hours after siRNA transfection, RNA was extracted using Trizol and reverse transcribed, followed by qPCR verification of gene silencing efficacy.
    The results demonstrate that the ​​siRNA/miRNA Transfection Reagent (HY-K2017)​​ achieves ​​significant silencing efficacy​​ against the ​​MALT1 gene​​ in ​​Caco-2 cells​​.

    • 描述及优势

    • 储存条件 & 期限

    • 包装

    • 文件资料

    描述及优势

    RNA 干扰 (RNA interference, RNAi)是指小分子双链 RNA 能够特异性地降解同源的 mRNA,从而抑制或关闭相应基因表达的现象。使用 RNAi 技术设计针对致病基因 mRNA 的小分子干扰 RNA (Small interfering RNA, siRNA),可以特异性剔除或关闭特定基因的表达。

     

    MCE siRNA/miRNA Transfection Reagent 是一种阳离子高分子聚合物的新型转染试剂,适用于 siRNA 和 miRNA 的转染。外源 siRNA/miRNA 与转染试剂形成稳定复合物,通过胞吞作用进入细胞,随后在胞质释放出 siRNA/miRNA,实现 siRNA/miRNA 的细胞转染。

     

    MCE siRNA/miRNA Transfection Reagent 具有以下优点:

    1. 适用范围广:可作用于多种细胞系,适合 siRNA/miRNA 介导的基因抑制实验。

    2. 转染效率高:高效转染原代细胞,细胞存活率高。

    3. 细胞毒性低:能较好平衡高转染效率与细胞活力,作用温和。

    4. 操作简便:实验方案简单快速,结果稳定可靠,转染后无需去除复合物或更换新鲜培养基。

    储存条件 & 期限

    -20℃, 1 年

    避免反复冻融

    包装
    Components HY-K2017-1 mL HY-K2017-5 mL
    siRNA/miRNA Transfection Reagent 1 mL 1 mL × 5
    文件资料

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    siRNA/miRNA转染试剂
    目录号:
    HY-K2017
    需求量: