2. Academic Validation
  3. Selective chemical imaging of static actin in live cells

Selective chemical imaging of static actin in live cells

  • J Am Chem Soc. 2012 May 23;134(20):8480-6. doi: 10.1021/ja211708z.
Lech-Gustav Milroy 1,  Stefano Rizzo,  Abram Calderon,  Bernhard Ellinger,  Silke Erdmann,  Justine Mondry,  Peter Verveer,  Philippe Bastiaens,  Herbert Waldmann,  Leif Dehmelt,  Hans-Dieter Arndt
Affiliations

Affiliation

  • 1 Department of Chemical Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany.
PMID: 22475347 DOI: 10.1021/ja211708z
Abstract

We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z