2. JAK/STAT Signaling Stem Cell/Wnt Cell Cycle/DNA Damage Apoptosis
  3. STAT CDK c-Myc Apoptosis
  4. STAT3-IN-53

STAT3-IN-53 (Compound L20) 是一种 STAT3 抑制剂，Kd 为 6.16 μM。STAT3-IN-53 可直接结合 STAT3 的 SH2 结构域，抑制 Y705 位点的磷酸化，且不影响总 STAT3 蛋白水平。STAT3-IN-53 可下调 cyclin-D1 和 c-Myc 的转录与表达。STAT3-IN-53 可诱导细胞周期阻滞并促进细胞凋亡 (Apoptosis)。STAT3-IN-53 对结直肠癌具有抗癌活性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

我们将采用定制合成服务的方式为您快速提供所需产品和技术服务

STAT3-IN-53

STAT3-IN-53 Chemical Structure

CAS No. : 3113051-34-3

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

STAT3-IN-53 相关抗体

Top Publications Citing Use of Products

查看 STAT 亚型特异性产品:

查看所有亚型
STAT3 STAT5 STAT6 STAT1

查看 CDK 亚型特异性产品:

查看所有亚型
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

STAT3-IN-53 (Compound L20) is a STAT3 inhibitor with a Kd value of 6.16 μM. STAT3-IN-53 binds directly to the SH2 domain of STAT3, inhibits phosphorylation at the Y705 site without affecting the total STAT3 protein level, and suppresses the IL-6/JAK/STAT3 pathway. STAT3-IN-53 downregulates the transcription and expression of cyclin-D1 and c-Myc. STAT3-IN-53 induces cell cycle arrest and promotes Apoptosis. STAT3-IN-53 exhibits anticancer activity against colorectal cancer[1].

IC50 & Target[1]

STAT3

6.16 μM (Kd)

体外研究
(In Vitro)

STAT3-IN-53 可直接与重组全长野生型 STAT3 蛋白结合，其 Kd 值为 6.16 μM[1]。
STAT3-IN-53 可在 HEK-BLUE-IL6 细胞中强效抑制 IL-6/JAK/STAT3 信号通路，其 IC50 为 0.89 μM[1]。
STAT3-IN-53 (96 h) 可强效抑制 HCT116、HT29、SW480、MC38 和 CT26 结直肠癌细胞系的增殖，其中对 HCT116 细胞的活性最强 (IC50 = 0.45 μM)[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

STAT3-IN-53 相关抗体:

Cell Proliferation Assay[1]

Cell Line: HCT116, HT29, SW480, MC38, CT26 colorectal cancer cell lines
Concentration: concentration gradient (for IC50 determination)
Incubation Time: 96 h
Result: Exhibited potent antiproliferative activity across all tested colorectal cancer cell lines, with IC50 values of 0.45 ± 0.05 μM (HCT116), 1.80 ± 0.10 μM (HT29), 9.34 ± 0.79 μM (SW480), 2.33 ± 0.48 μM (MC38), and 5.14 ± 0.84 μM (CT26).

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 0.5, 2.5 μM
Incubation Time: 24 h
Result: Dose-dependently suppressed STAT3 phosphorylation at the Y705 residue without altering total STAT3 protein levels.
Did not inhibit phosphorylation of STAT6 or SRC.
Dose-dependently downregulated cyclin-D1 expression.
体内研究
(In Vivo)

STAT3-IN-53 (5-20 mg/kg；腹腔注射；每日一次；12 天) 可在 C57BL/6J 小鼠中对皮下 MC38 结肠腺癌的生长呈现剂量依赖性抑制[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (male, SPF grade, 18-20 g, subcutaneous implantation of MC38 colon adenocarcinoma tumor fragments)[1]
Dosage: 5 mg/kg; 20 mg/kg
Administration: i.p.; once daily; 12 days
Result: Achieved a tumor growth inhibition (TGI) rate of 53.6% at 5 mg/kg.
Achieved a tumor growth inhibition (TGI) rate of 59.8% at 20 mg/kg.
Suppressed STAT3 Y705 phosphorylation in tumor tissues at 20 mg/kg.
Downregulated Cyclin-D1 and c-Myc protein expression in tumor tissues at 20 mg/kg.
Caused no treatment-related adverse events or body weight loss exceeding 10% during the 12-day dosing period.
分子量

678.73

Formula

C33H33F3N8O3S
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

STAT3-IN-53 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STAT3-IN-533113051-34-3STATCDKc-MycApoptosisCyclin dependent kinaseMycmurine subcutaneous xenograft modelIL-6/JAK/STAT3 pathwayY705SH2 domaincolorectal cancer cellsSTAT3HEK-BLUE-IL6 cellsHCT116 cellscyclin-D1Inhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
STAT3-IN-53
目录号：
HY-181514
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z