2. Epigenetics Cytoskeleton Cell Cycle/DNA Damage Apoptosis
  3. Histone Methyltransferase PAK Bcl-2 Family
  4. WX2-43

WX2-43 是一种 PRMT5-KLF4 抑制剂 (采用 biotin-WX2-43 与 GST-PRMT5 检测的 Kd 为 11.0 nM)。WX2-43 结合于 PRMT5 上第 L400 至 M500 位氨基酸之间的区域。WX2-43 可有效阻断 KLF4 与 PRMT5 的相互作用，并抑制 KLF4 的甲基化。WX2-43 可降低 p21 水平并升高 Bax 水平。WX2-43 可抑制三阴性乳腺肿瘤的进展。WX2-43 可用于三阴性乳腺癌和结直肠癌的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

WX2-43

WX2-43 Chemical Structure

CAS No. : 723754-14-1

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

WX2-43 相关抗体

Top Publications Citing Use of Products

查看 Histone Methyltransferase 亚型特异性产品:

查看所有亚型
EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D ASH1L/KMT2H SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

查看 PAK 亚型特异性产品:

查看所有亚型
PAK1 PAK2 PAK3 PAK4 PAK5 PAK6

查看 Bcl-2 Family 亚型特异性产品:

查看所有亚型
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

WX2-43 is a PRMT5-KLF4 inhibitor (Kd of 11.0 nM: biotin-WX2-43 with GST-PRMT5). WX2-43 binds to the region between amino acids L400-M500 on PRMT5. WX2-43 efficiently intercepts the interaction between KLF4 and PRMT5, and blocks KLF4 methylation. WX2-43 decreases p21 levels and increases Bax levels. WX2-43 suppresses triple negative breast tumor progression. WX2-43 can be used in the research of triple-negative breast cancer and colorectal cancer[1][2].

IC50 & Target[1]

PRMT5

11.0 nM (Kd)

Bax

 

体外研究
(In Vitro)

WX2-43 (生物素化 WX2-43；0.1 nM-10 μM；孵育 2 小时) 与 PRMT5 结合，其 Kd 值为 11.0 nM[1]。
WX2-43 (0.03-10 μM；作用 24 h) 以 KLF4 依赖的方式抑制经顺铂处理的 MEFs 细胞活力[1]。
WX2-43 在三阴性乳腺癌 (TNBC) 细胞系中的活性更强 (EC50 0.44-0.96 μM)，高于正常乳腺上皮细胞系 (EC50 3.62-23.34 μM)[1]。
WX2-43 可在二维 (2D) AKPS 培养体系中诱导间充质样表型，改变克隆形态、大小及肌动蛋白细胞骨架，并降低 Ki-67+ 细胞的频率[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

WX2-43 相关抗体:

Cell Viability Assay[1]

Cell Line: KLF4+/+ MEFs, KLF4-/- MEFs
Concentration: 0, 0.03, 0.1, 0.3, 1, 3, 10 μM (WX2-43); 5 μM (cisplatin)
Incubation Time: 24 h
Result: Reduced viability in KLF4+/+ MEFs in a dose-dependent manner; showed no effect on viability in KLF4-/- MEFs.

Western Blot Analysis[1]

Cell Line: MDA-MB-231
Concentration: 0, 0.1, 1, 10 μM
Incubation Time: 24 h
Result: Decreased KLF4 protein levels and p21 levels and increased Bax levels in a dose-dependent manner.
体内研究
(In Vivo)

WX2-43 (40-80 mg/kg；腹腔注射；每周 2 次；持续 4 周) 可抑制裸鼠体内三阴性乳腺癌的肿瘤进展[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Crl: Nu-Foxn1(nu) (female, 8 weeks old)[1]
Dosage: 40 mg/kg; 80 mg/kg
Administration: i.p.; twice weekly; 4 weeks
Result: Reduced tumor size and weight by approximately 40% at 40 mg/kg compared to vehicle; Reduced tumor size and weight by approximately 60% at 80 mg/kg compared to vehicle; Confirmed reduced KLF4 protein levels in treated groups via western blotting.
分子量

288.82

Formula

C17H21ClN2
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

WX2-43 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WX2-43723754-14-1Histone MethyltransferasePAKBcl-2 Familyp21 activated kinasescolorectal cancertriple negative breast cancerKi-67AKPS colorectal cancer organoidsnude miceKLF4MDA-MB-231 cellsZEB1PRMT5MEFsInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
WX2-43
目录号：
HY-W072962
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z