Tris-maleate缓冲液怎么配置？

<p>20mM Tris-Malate Buffer:</p> <p>24.2 g of tris(hydroxymethyl)aminomethane plus 23.2 g of maleic acid or 19.6 g of maleic anhydride in 1000 ml of distilled water</p>

Tris-maleate：

三羟甲基氨基甲烷马来酸酯

1S\/2C4H11NO3.C4H4O4\/c2*5-4(1-6,2-7)3-8;5-3(6)1-2-4(7)8\/h2*6-8H,1-3,5H2;1-2H,(H,5,6)(H,7,8)\/b;;2-1-

其他名称：

MONO[TRIS(HYDROXYMETHYL)AMINOMETHANE]MALEATE;

TRIZMAMALEATE;

TRIS(HYDROXYMETHYL)AMINOMETHANEMALEATE;

TRISMALEATEBUFFER;

TRISMALEATESALT;

TRISMAT(TM)MALEATE;

[2-HYDROXY-1,1-BIS(HYDROXYMETHYL)ETHYL]AMMONIUMHYDROGENMALEATE