如何配DMEM培养基

这里是一份G领严差温值体美座IBCO的低糖DMEM粉剂配制说明（普通高糖的DMEM培养基配法是一样的）：
TO PREPARE 1*L均IQUID
1. Measrue out 5% less distilled water than desired total volume of medium using a mixing container that is as close to the final volume as possible.
2. Add powdered medium to 15 to 30℃ (room temperature)water with gentle stirring. (Do not h落究在死攻留eat water)
3. Rinse out inside of package to remove all traces of powder七需.
4. Add 3.来自7g of NaHCO3 per liter of medium.
5. Dilute to a desired volume with water. St龙奏足首写觉能ir until dissolved. (Do not over-mix)
6. Adjus若美聚吗效面t pH of medium to 0.20.3 belo谁钱鸡却候居商司w desired final working pH* use of IN NaOH or IN HCl is recommended. (Add slo360问答wly with stirring) After pH has been adjuste keep containe鱼下轴晶明黑抓女传输穿r closed u工民布神烟热通硫动领ntil medium is filtered.
7. Sterilize immediately by membrane filtration. (Positive pressure recommended) *pH unite will usually rise 0.1-0.3upon filtration.
另外，在李玲、李雪峰编著的《细胞生物学实验》中配制方法如下：
1 制备新鲜三蒸水或Millipore超纯水。
2 称取所需量的干粉培养基，加入约终体积一半的三蒸水各具根中；若配制一个包装的培养液，在将整个包装的干粉倒入三蒸振除望致员有烟水后，需用水洗包装袋内面2次，倒入培养液中，以保证所有干粉都溶解成培养液。磁很沙错若明力搅拌或人工搅拌使之完全溶解。
3 根据包装袋上的要求补加所安局米刘的策需量的碳酸氢钠；根据实验需要，添HEPES（蔽谈档5-20mmol/L）、谷氨酰胺和其他特殊物质。
灯卫维际4 加水定容到宏乱终体积。
5 必要时用1 mol/L 盐酸和1 mol/L ****调节pH。
6 用无菌0.22殖雨测末威杂现um滤膜过滤除侍伏菌，分装于无菌血清瓶中，4℃冰箱保存。 配制好的培养液用前加入100U/mL青霉素和100U/mL链霉素，并根据需要加入血清（5%-2说量九群经族未历演0%）。