pcr产物电泳后为何条带过宽,而且有的条带清晰,有的则不清晰

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回答者:网友
You can try either of the following or both: 1.Clean the PCR mix before run it using Qiagen kit, it may be better. 2. heat the PCR mix at 60C for 5 min. make sure your agarose gel is made correctly.

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条带过宽或者不清晰是因为DNA被降解了,或者是凝胶没做好
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