方法
Wester已创站永迫与九n blotting
Wester福呀居n blotting法检测P53靶向抑制剂-EGFR对人结肠癌细胞株（H丝火区从耐解美T-29,colo205）中，p53/p27信号通路下游关键因子p27的蛋白表达情况，并将人结肠癌细胞株区分为p27高表达的细胞株和低表达的细胞株。
Methodology
Western blotting
The protein exp日克香后足ression of t状击艺顺刑则he p53/p2快实精延另命房顾7 signal pathway downstream key factor p2主跳溶护套爱星议夫指作7 in the treatment of human colon carcinoma cell strain（HT-29,colo205）b喜y P53 targeted inhibitor EGFR was examined by the Western blotting method, and the human colon carcinoma cell strains were separated int血检导判买四袁承本o p27 high expression and low expression cell strains.
2. MTT和RT-PCR
MTT法测定EGFR热走目析火术对结肠癌细胞转染前后抑制率的变化，RT-PCR法测定p27沉默前后，mRNA的表达量的变化,根据实验结果进行统计分析。
2见远天形心. MTT and RT-PCR
While the detect句存价ion of the changes in inhibition rate of EGFR before and after colon carcinoma cell transfection was conducted by the MTT met停律游话hod, the RT-PCR method w亲然控层as used to detect mRNA expression level changes before and after P27 silencing, and then a statistic analysis was conducted based on the experiment res交部架候至庆材ults.
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