流式细胞仪怎样检测小鼠脾脏中Th17溶弦热立盟沉烟较补细胞和Treg细专胞数量具体步骤，求高人指导啊

ヘルパーT 细胞パラダイム―Th来自17细胞とTreg细胞による疾患形成と360问答制御―
野间刚
Helper T cell paradigm :T父站德山h17 and regulatory T cells involved in autoimmune in‰ammatory
disorders,p提游也卫athogen defen连布重基se and allerg吸践系单场乎岩异参定元ic diseas导es.
Takeshi NOMA,MD,PhD
Kitasato Uni航渐房曲易粉更右versity Graduate School of Medical Science,Department of Pediatrics
1-15-1 Kitasato,Sagamih围笑造已块征江攻加ara,Kanagawa,Japan 2288555
文献里有图