用这两种引物：BcaBEST Sequencing Primer M13-47和煤存本视BcaBEST Sequencing Primer RV-M
Vector size (bp) 2692
Cloning Site 425
LacZ α-peptide 146-475
Ampicillin resistance gene 1632~2492
pUC origin 873-流会远属局立织那小球亚1461
primer binding sites：
BcaBEST Seq负uencing Primer M1县补日3-47 binding site 352-375
BcaBEST Sequencing Primer RV-M binding site 484-507
