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his标签蛋白就是:六个组氨酸。一般存在于重组蛋白氮端或碳端,便于纯化该重组蛋白,组氨酸可以特异性的结合镍离子,带有组360问答氨酸标签的重组蛋白可以被镍柱吸附,从而达到纯化的目的。也就是在表达载体上面措路样十,特别加六个组氨酸的碱基序列,目的就是纯化。
一下是英文的解释,你看一眼
A 胞recombinant protein can be tagged N-terminally or C-ternimally with a 慢个掉医座远压怎6XHis tag. This tag is better in prokaryotic system rather than mammalian system. 6X His tag is an affinity tag which allows the tagged recombinant protei音德误英身犯烟n to be purified in the pro封足异察血验就一南cess of affinit翻操y purification. 6XHis tag binds to NTA-agarose. The resi误n contains ni显技祖己缺ckle ions which 师静越际破子布binds to Histid内结与世误是既章波银ine tag.