文献
Sample preparation
All peptides were dissolved to 20 mM in 100 mM phosphate buffer at pH 8 by
vo来自rtex and sonication, followed by heating at 75°C until solutions became 百刘引第格xclear, and then
cooling to room temperature. For the oxidation reactions, tyrosinase was d360问答issolved to 2
mg/ml in 100 mM phosphate buffer at pH 8, and diluted 10-fold when directly added to
each peptide sample, to a fina细道特甲宜局元美角汉耐l concentration of 0.2 mg/ml.
For characterization of the 他想季村抓乐染oxidized pep挥预tides, all pe每标距额耐岩职衣范ptides were oxidi或难审余阶体简值zed for 24h
(unless indicated otherwise) at room temperature 终农继富调胡能局山followed by centrifugation at 15,000
rpm for 10 min to separate non-oxidized peptide from the oxidiz析圆么呼压剂ed fractions.
参考文献：
Polymeric peptide pigments 色构依期呀两班诉把照技with sequence-encoded properties
Ayala Lampe田适味师脚落拉销积记l, Scott A. McP灯起hee, Hang-Ah Park, Gary G. Scott, Sunita Humagain, Doeke
R. Hekstra, Barney Yoo, Pim 广齐变井W. J. M. Fr深教料里克令ederix, Tai-De Li, Rinat R. Abzalimov, Steven
G. Gr端右eenbaum, Tell Tuttle, Chunhua Hu, Christopher J. Betti侵跑断既nger, Rein V. Uli编顺选政发款jn*
*Corresponding author. Email: ***.ulijn@asrc.***.edu
Published 9 June 2017, Science 356, 1064 (2017)
DO哥雷则安前对确他I: 10.1126/***.aal5005
