UV-method for the determination of D-Gluconic Acid and 
D-Glucono-δ-lactone in foodstuffs, beverages and other 
materials

Principle:
(gluconate kinase)
(1) D-Gluconate + ATP → gluconate-6-phosphate + ADP

(gluconate-6-phosphate dehydrogenase)
(2) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + 
NADPH + CO₂ + H⁺

(pH 11)
(3) D-Glucono-δ-lactone + H₂O → D-gluconate

Kit size: 60 assays (manual) / 600 (microplate)
/ 600 (auto-analyser)
Method:  Spectrophotometric at 340 nm
Reaction time:  ~ 6 min
Detection limit: 0.5 mg/L
Application examples:
Wine, meat, processed meat (e.g. additives), fruit juice, dairy products,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by ISO,
DIN and GOST