ITS is a mixture of insulin, transferrin, and selenium. It is a general cell supplement designed for use in classical media such as DMEM, RPMI-1640 and nutrient media such as Ham’s F-12, DMEM/F-12. ITS enhances cell proliferation and decreases the serum requirement of many cell types [1, 2, 3]. When ITS is used with a low percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.

ITS is prepared in Hank’s Balanced Salt Solution (HBSS) without phenol red.

Formula

Insulin 1.0 mg/ml

Apo-Transferrin 1.0 mg/ml

Sodium Selenite 3.4 µM