Tryptone is a pancreatic digest of casein. It can be used

in any formulation where a pancreatic or tryptic digest

of casein is specified. Casein is the main protein of

milk and is a rich source of amino acid nitrogen. The

profile shows a broad spread of peaks throughout the

molecular weight range characteristic of a pancreatic

digest. This hydrolysate is often mentioned in published

works, either as a constituent of culture media for

metabolic or growth studies, or for other purposes where

high performance and uniformity of composition are of

paramount importance. It has a high tryptophan content

and is therefore used in media for testing the indole

reaction.
Tryptone can detect `flat-sour’ or `sulphide’ spoilage

organisms in the canning industry and is also used in

sterility testing media.
It is a constituent of media used in fermentation

processes to produce antibiotics, extra-cellular protein,

interferon and diphtheria toxoid.