ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) 

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• Product No.SEA143Hu
• Organism SpeciesHomo sapiens (Human) Same name, Different species.
• Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Test MethodDouble-antibody Sandwich
• Assay Length3h
• Detection Range15.6-1000pg/mL
• SensitivityThe minimum detectable dose of this kit is typically less than 6.2pg/mL.
• SpecificityThis assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA).
  No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
• ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
• DownloadInstruction Manual
• Format48T96T96T*596T*1096T*100
• FOBUS$ 560 
  For negotiated price and more details, please contact local distributors!

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.