| 英文名 | BD MitoScreen (JC-1) |
| 保存条件 | 低温 |
| 规格 | 100 Tests |
Store the unopened vials of JC-1 and 10× Assay Buffer at 2° – 8°C.
Barbieri, D., M.P. Abbracchio, S. Salvioli, D. Monti, A. Cossarizza, S. Ceruti, R. Brambilla, F. Cattabeni, K.A. Jacobson, and C. Franceschi. 1998. Apoptosis by 2-chloro-2’-deoxy-adenosine and 2-chloro-adenosine in human peripheral blood mononuclear cells. Neurochemi. Int. 32:493-504.
Cossarizza, A., M. Baccarani-Contri, G. Kalashnikova, and C. Franceschi. 1993. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cationic 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem. Biophs. Res. Comm. 197:40-45.
Facompre, M., N. Wattez, J. Kluza, A. Lansiaux, and C. Bailly. 2000. Relationship between cell cycle changes and variations of the mitochondrial membrane potential induced by etoposide. Mol. Cell Biol. Res. Comm. 4:37-42.
Gravance, C.G., D.L. Garner, J. Baumber, and B.A.Ball. 2000. Assessment of equine sperm mitochondrial function using JC-1. Theriogenology 53:1691-1703
Mantymaa, P., T. Sitonen, T. Guttorm, M. Saily, V. Kinnula, E-R. Savolainen, and P. Koistinen. 2000. Introduction of mitochondrial manganes superoxide dismutase confers resistance to apoptosis in acute myeloblastic leukemia cells exposed to etoposide. British J. Haematol. 108:574-581.
Mathur, A., Y. Hong, B.K. Kemp, A.A. Barrientos, and J.D. Erusalimsky. 2000. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes. J. Cardiovascular Res. 46:126-138.
Petit, P.X., H. Lecoeur, E. Zorn, C. Dauguet, B. Mignotte, and M-L. Gougeon. 1995. Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J. Cell Biol. 130:157-167.
Salvioli, S., Ardizzoni, A., Franceschi, and A. Cossarizza 1997. JC-1, but not DiOC6(3) orrhodamine 123, is a reliable fluorescent probe to assess ∆ψ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS 411:72-82.
Salvioli, S.,k J. Dobrucki, L. Moretti, L. Troiano, M.G. Fernandez, M. Pinti, J. Pedrazzi, C. Franceshi, and A. Coassarizza. 2000. Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: Analysis at the single cell and single organelle level. Cytometry 40:189-197.
The BD™ MitoScreen Kit is designed for use in flow cytometry. It consists of two reagents, JC-1 and 10× Assay Buffer. JC-1 excites at 488 – 490 nm. The monomeric form emits at 527 nm; J-aggregates emit at 590 nm. There are enough reagents to test 100 samples by flow cytometry using the protocol provided.
