Introduction
Angiotensin II, a main effector peptide in the renin-angiotensin system, acts as a growth promoting and angiogenic factor via type-1 angiotensin II receptors (1). Angiotensin II is suggested to be involved in the regulation of cell proliferation (2), angiogenesis (3), inflammation (4), and cancer (1).

Principle of the Assay The AssayMax Human Angiotensin II ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human angiotensin II in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures angiotensin II in less than 5 hours. A polyclonal antibody specific for angiotensin II has been pre-coated onto a 96-well microplate with removable strips. The angiotensin II in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for angiotensin II, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.