应用:
| μ-Slide参数:
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| 材质 | SiO2 (glass) | ||
| 厚度 | 0.3 μm (300 nm) | ||
| 膜尺寸 | 2 mm x 2 mm | ||
| 可选孔径 |
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生长在3 μm膜上的内皮细胞，图示为免疫荧光与相差成像的叠加图。DAPI (蓝色), VE-Cadherins (绿色), F-Actin (红的).
生长在3 μm ibiPore膜上的成纤维细胞。使用4倍物镜拍摄的相差成像。
| 膜种类 | 应用 | 细胞示例 |
| 0.5 μm pores, high porosity (20%) | Permeability and transport studies, co-culture models. With this model, cell migration is not possible. | Lung cells, epithelial cells |
| 3.0 μm pores, low porosity (5%) | Transendothelial migration | Leukocytes (neutrophils, T-cells) |
| 5.0 μm pores, low porosity (5%) | Invasion, migration | Monocytes, macrophages, lymphocytes |
| 8.0 μm pores, low porosity (5%) | Invasion, migration | Tumor and cancer cells, endothelial and epithelial cells, fibroblasts, osteoblasts, melanoma, glioma |
The bigger the cell – the bigger the recommended pore size.
μ-Slide Membrane ibiPore Flow的剖面图 | μ-Slide Membrane ibiPore Flow组成结构
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内皮屏障 在玻璃网筛膜一侧培养单层细胞并成像。细胞可以在静止或者流动剪切力条件下培养. | 2D跨内皮迁移，在玻璃网筛的一侧培养单层细胞。可以观察悬浮的白细胞在流动状态下的滚动、粘附以及侵袭情况。 | 3D凝胶基质中的细胞迁移，在流动状态下，观察白细胞的滚动、粘附以及向3D凝胶基质中肿瘤细胞方向的迁移情况。 | |||||
共培养及细胞屏障检测，在玻璃网筛的两侧分别培养单层细胞。信号传递，共培养以及迁移实验都是可以检测的。 | 极性检测，3D凝胶基质中的化学因子可以导向在膜另一侧培养的单层细胞的极性发生。 | 气液界面下的皮肤及肺模型，气液界面条件下的极性化的细胞实验。 | |||||
3 μm pores | 3 μm single pore |
0.5 μm pores | 0.5 μm single pores |
| Cat. No. | Description | Pcs./Box | |
|---|---|---|---|
| 85016 | µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 0.5 µm porous glass membrane, 20% porosity, sterilized | 10 | |
| 85026 | µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 3.0 µm porous glass membrane, 5% porosity, sterilized | 10 | |
| 85036 | µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 5.0 µm porous glass membrane, 5% porosity, sterilized | 10 | |
| 85046 | µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 8.0 µm porous glass membrane, 5% porosity, sterilized | 10 | |
| 85016-S | µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 0.5 µm porous glass membrane, 20% porosity, sterilized | 2 | |
| 85026-S | µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 3.0 µm porous glass membrane, 5% porosity, sterilized | 2 | |
| 85036-S | µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 5.0 µm porous glass membrane, 5% porosity, sterilized | 2 | |
| 85046-S | µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 8.0 µm porous glass membrane, 5% porosity, sterilized | 2 | |
