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  3. Platinum Green Hot Start PCR Master Mix (2X)抗体热启动酶

Platinum Green Hot Start PCR Master Mix (2X)抗体热启动酶

¥500
Invitrogen
进口原装
2021-07-13 04:47
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上海恒斐生物科技有限公司

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上海恒斐生物科技有限公司
刘鑫
17317793691 021-61843644
845206284@qq.com
845206284
产品属性
保存条件Store at -20°C in a non-frost-free freezer.
英文名Platinum™ Green Hot Start PCR Master Mix (2X)
数量大量
供应商恒斐生物
CAS号相关应用： Capillary Electrophoresis Sequencing & Fragment Analysis | Genotyping & Genomic Profiling | PCR | 甲基化分析
规格50 reactions
产品说明

Platinum Green Hot Start PCR Master Mix (2X)抗体热启动酶

Polymerase:Platinum™ Taq DNA Polymerase
Hot Start:Built-In Hot Start
Fidelity (vs. Taq):1 X
GC-Rich PCR Performance:Medium
Reaction Format:SuperMix or Master Mix
Product Overhang:3'-A
Reaction Speed:Standard
Final Product Size(s):5 kb or less
Product Size:50 reactions
Shipping Condition:Dry Ice

Platinum Green Hot Start PCR Master Mix (2X)抗体热启动酶
内容及储存

Each box contains:
• 1.25 mL Platinum Green Hot Start PCR 2X Master Mix
• 1.25 mL Platinum GC Enhancer
• 1.25 mL water, nuclease-free

Store at -20°C in a non-frost-free freezer.
Invitrogen™ Platinum™ Green Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. Platinum Green Hot Start PCR Master Mix retains all the features of Platinum Taq DNA Polymerase: specificity, robustness and reliability. Platinum Green Hot Start PCR Master Mix is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

Product features:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Direct gel loading with green PCR master mix
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, theTaq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum TaqDNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity).

Using Platinum Green Hot Start PCR Master Mix
Convenient Platinum Green Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water. The two tracking dyes in Platinum Green Hot Start PCR Master Mix enable direct gel loading of PCR products, thus eliminating tedious steps of dye addition. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, including DNA sequencing, ligation, and restriction digestion. Platinum Green Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Green Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.
货号
单位规格
单价 (CNY)数量
13001012
也被称为 13001-012
50 reactions
596.00
13001013
也被称为 13001-013
200 reactions
2,237.00
13001014
也被称为 13001-014
1000 reactions
8,851.00

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