| 产品名称 | anti- SMAD4 antibody |
| 货号 | FNab07997 |
| 规格 | 100µg |
| 状态 | liquid |
| 纯化方法 | Immunogen affinity purified |
| 纯度 | ≥95% as determined by SDS-PAGE |
| 宿主 | Rabbit |
| 亚型 | IgG |
| 存储 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| 背景介绍 | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein(BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements(SBEs)(5'-GTCT/AGAC-3') within BMP response element(BMPRE) of cardiac activating regions(By similarity). Common SMAD(co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta(transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| 免疫原 | SMAD family member 4 |
| 别称 |
| 分子量 | 48 kDa |
| Uniprot ID | Q13485 |
| 适用种属 | Human, Mouse, Rat |
| 验证应用 | ELISA, WB, IHC |
| 建议稀释比例 | WB: 1:500-1:2000; IHC: 1:20-1:200 |
| 图片 |  | Immunohistochemistry of paraffin-embedded human placenta tissue slide using FNab07997(SMAD4 Antibody) at dilution of 1:50 |
 | HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab07997(SMAD4 antibody) at dilution of 1:600 |
| 注意事项 | SMAD4 antibody|SMAD4抗体仅供科研使用 |
